| 成牙本质细胞中上游刺激因子1对骨桥素表达调控的研究 |
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| 引用本文： | 吴礼安,文玲英,杨富生,王小竞. 成牙本质细胞中上游刺激因子1对骨桥素表达调控的研究[J]. 中华口腔医学杂志, 2007, 42(11): 663-664 |
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| 作者姓名： | 吴礼安 文玲英 杨富生 王小竞 |
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| 作者单位： | 第四军医大学口腔医学院儿童口腔科,西安,710032 |
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| 摘 要： | 目的检测成牙本质细胞中上游刺激因子1（upstream stimulatory factor 1，USF1）对骨桥素表达的影响。方法培养成牙本质细胞MDPC-23，稳定转染PCMV-USF1和酸性-USF（A-USF）质粒，提取总RNA，半定量反转录聚合酶链反应检测骨桥素的表达水平，并对结果进行统计学分析。结果筛选出稳定转染USF1和A-USF的抗性克隆，USF1、A-USF转染组和对照组骨桥素相对灰度比值分别为：60．33％±4．51％、229．33％±7．09％、110．00％±15．62％，转染组与对照组差异有统计学意义（P〈0．01）。结论USF1可调控成牙本质细胞骨桥素mRNA转录，该作用被A-USF部分阻断。
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| 关 键 词： | 牙本质生成 逆转录聚合酶链反应 成牙本质细胞 |
| 修稿时间： | 2007-01-29 |
Regulation of osteopontin expression in odontoblasts by upstream stimulatory factor 1 |
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| WU Li-an,WEN Ling-ying,YANG Fu-sheng,WANG Xiao-jing. Regulation of osteopontin expression in odontoblasts by upstream stimulatory factor 1[J]. Chinese journal of stomatology, 2007, 42(11): 663-664 |
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| Authors: | WU Li-an WEN Ling-ying YANG Fu-sheng WANG Xiao-jing |
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| Affiliation: | Department of Pediatric Dentistry, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China. |
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| Abstract: | OBJECTIVE: To investigate the regulation effects of upstream stimulatory factor 1 (USF1) on osteopontin expression in odontoblasts. METHODS: Odontoblast MDPC-23 was cultured and stably transfected with PCMV-USF1 or A-USF plasmids. Total RNA was extracted and osteopontin expression examined by semi-quantitative RT-PCR. Gray value of osteopontin was measured and statistic analysis performed. RESULTS: Clones of stable PCMV-USF1 and A-USF plasmids transfection were obtained. Compared with the control, osteopontin was upregulated in PCMV-USF1 transfection group, and downregulated in A-USF transfection group. CONCLUSIONS: Upstream stimulatory factor 1 could regulate the osteopontin expression in odontoblasts, which could be blocked partly by A-USF. |
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