| LPS调节U937细胞上B7-H1表达的初步研究 |
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| 引用本文： | 黄钢,姜曼,白云. LPS调节U937细胞上B7-H1表达的初步研究[J]. 免疫学杂志, 2006, 22(5): 480-483 |
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| 作者姓名： | 黄钢 姜曼 白云 |
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| 作者单位： | 第三军医大学基础医学部,医学遗传学教研室,重庆,400038;第三军医大学基础医学部,免疫学教研室,重庆,400038 |
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| 基金项目： | 国家自然科学基金;国家重点基础研究发展计划(973计划) |
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| 摘 要： | 目的了解脂多糖（LPS）刺激后U937细胞上B7-H1的表达变化情况。方法培养U937细胞，用荧光半定量实时PCR和流式细胞仪技术，分别观测未刺激和用LPS刺激的U937细胞B7-H1mRNA与蛋白的表达情况。结果未经刺激的U937细胞组成性表达B7-H1，LPS刺激可显著增强B7-H1基因mRNA的转录和蛋白的表达。结论LPS在转录与翻译两个环节均可上调U937细胞B7-H1的表达水平。
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| 关 键 词： | U937 脂多糖 B7-H1 表达调节 |
| 文章编号： | 1000-8861（2006）05-0480-04 |
| 收稿时间： | 2005-12-19 |
| 修稿时间： | 2006-06-14 |
Expression modulation of B7-H1 in U937 cells after LPS stimulation |
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| HUANG Gang,JIANG Man,BAI Yun. Expression modulation of B7-H1 in U937 cells after LPS stimulation[J]. Immunological Journal, 2006, 22(5): 480-483 |
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| Authors: | HUANG Gang JIANG Man BAI Yun |
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| Affiliation: | Department of Medical Genetics, College of Basic Medicine, Third Military Medical University, Chongqing 400038, China |
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| Abstract: | Objective To investigate the expression of human B7-H1 in U937 cells after LPS stimulation. Methods The expression of B7-H1 gene was detected by flourescence semiquantitative real-time PCR assay and FCS assay before and after stimulation. Resuits Unstimulated U937 cells expressed B7-H1 constitutively. LPS stimulation could upregulated the levels of B7-H1 mRNA and protein in U937 cells. Conclusion LPS can upregalate the constitutive expression of B7-H1 at the levels of mRNA and protein. |
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| Keywords: | U937 B7-H1 |
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