抗内毒素类脂A单克隆抗体对烧伤脓毒症大鼠的保护作用

采用我所研究制备的抗内毒素类脂 A 单克隆抗体(mAb)观察了其对攻菌后动物的保护作用,并采用30%Ⅲ度 TBSA 烧伤大鼠模型注射内毒素,模拟临床烧伤脓毒症,旨在为临床防治提供理论依据。实验动物分烧毒组、治疗组及对照组,于伤后不同时相点观察血浆 LPS、TNF 的动态变化,同时作内脏的光镜及电镜形态学检查。结果表明:mAb 除在体外可与多种 G~-杆菌及纯化的内毒素反应外,细菌攻击前后注入 mAb 均能有效地提高感染动物的存活率,并可明显降低模拟烧伤脓毒症大鼠血中内毒素,减少 TNF 的分泌。光、电镜观察证实,可减轻机体的损害效应。提示:抗类脂 A 单克隆抗体是一种有效的治疗烧伤脓毒症的免疫制剂。

Protective effect of lipid A monoclonal antibody against burn sepsis in rats.

A lipid A monoclonal antibody(mAb) was prepared and it was used to study its protec- tive effect against burn sepsis.Wistar rats were inflicted with 30% TBSA third degress burn,and they were given LPS to mimic early sepsis after burn.The rats were divided randomly into burn with LPS, monoclonal antibody treatment ,and control groups.The levels of endotoxin,tumor necrosis factor,light and electron microscopic studies of the morphological changes in the liver were studied.The results showed that the anti-lipid A monoclonal antibody demonstrated capacity to cross-react with several Gram-negative bacteria and their endotoxins.The mAh improved the survival rate of rats and decreased the levels of endotoxin and TNF as well as the liver damage significantly.