大鼠脊髓P物质对绵羊红细胞引起的体液免疫反应的影响

以大鼠脾脏空斑形成细胞(PFC)技术作为反映绵羊红细胞(SRBC)引起的体液免疫反应的指标,用放射免疫测定法测定脊髓P物质(SP)的含量,高效液相色谱(HPLC)与电化学检测器连用测定儿茶酚胺(CA)的含量,对大鼠脊髓SP对SRBC体液免疫反应的影响及其可能机制进行了探讨。结果提示,脊髓后角SP对SRBC引起的胸腺依赖性体液免疫反应起抑制作用,这种作用可能与脊髓后角SP对胸腺活动的影响有关,免疫反应高峰期脊髓后角SP含量增多可反馈性地抑制体液免疫反应,使之不至于过强。此外,从CA及SP测定结果看,脊髓侧角SP参与调节SRBC引起的体液免疫反应的可能性不大。

Effects of Substance P in Rat Spinal Cord on the Humoral Immune Response to SRBC

The effects of substance P (SP) in rat spinal cord on the humoral immune response to sheep red blood cells (SRBG) were investigated by the hemolytic plaque-forming cell (PFG) technique. Radioimmunoassay was used for assessing SP content. Gatecholamines contents were measured by high performance liquid chromatography (HPLG) with electrochemical-detection. The results suggest that SP in the spinal dorsal horn, but not in the lateral horn, could inhibit the thymus-dependent humoral immune response to SRBG, and this inhibitory effect might be related to the influence of dorsal horn SP on thymic activity. Increased SP content in the spinal dorsal horn at the peak of a humoral immune response might suppress the immune response and play a negative feedback role, preventing excessive immune response.