银杏叶制剂对正常脑温大鼠脑缺血治疗的实验研究

目的：观察常温下银杏叶提取物（GBF）对脑缺血组织抗氧化酶，脂质过氧化物及脑含水量的影响。方法：24只Wistar大鼠随机分为假手术组，脑缺血对照组及GBE治疗组（n=8)，制备正常脑温大鼠大脑中动脉缺血／再灌注模型，观察脑缺血组织超氧化物歧化酶（SOD），谷胱甘肽过氧化物酶（GSH－Px)，还原型谷胱甘肽（GSH），丙二醛（MDA）含量及脑含水量变化。结果：对照组SOD，GSH－Px,GSH水平低于手术组，治疗组高于对照组（P＜0．01或P＜0．05），对照组MDA水平高于假手术组，治疗组低于对照组（P＜0．01），对照组脑含水量高于假手术组，治疗组低于对照组（P＜0．01或P＜0．05），结论：正常脑温下，国产GBE能抑制脑缺血时自由基的过多产生和脂质过氧化反应，减轻脑水肿和血脑屏障破坏，保护脑缺血组织。

Study on the Treatment of Ischemia and Reperfusion Injure of Mouse in Normal Brain Temperature with GBE

Objective:To study the effect of Ginkgo biloba extract(GBE) on antioxidase,lipid peroxidation and water content in brain in normal temperature.Method:24 mice were randomly divided into pretending operation group,the control group and the treatment with GBE group(n=8).The contents of SOD,GSH Px,GSH,MDA and water in brain were determined.Result:SOD,GSH Px.GSH in the control group were lower than that in pretending operation group,it in treatment was higher than that in the control group(P<0 01).MDA in the control group was higher than that in the pretending group,it in the treatment group was lower than that in the control group(P<0 01).Conclusion:In normal temperature,free radical production and lipid peroxidation could be inhibited.And cerebral edema could be abated.