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| 白术多糖含量两种测定方法的比较 | |
| 引用本文： | 谭敏,邱细敏. 白术多糖含量两种测定方法的比较[J]. 现代中药研究与实践, 2012, 0(2): 73-75 |
| 作者姓名： | 谭敏 邱细敏 |
| 作者单位： | 常德职业技术学院;湖南师范大学医学院 |
| 基金项目： | 湖南省自然科学基金资助课题(编号08jj3024);长沙市科技管理局(K069062-12);湖南省医药卫生科研资助课题(B2007119) |
| 摘 要： | 目的通过白术多糖的含量测定研究,比较苯酚-硫酸和蒽酮-硫酸两种常用测定多糖的方法。方法通过精密度试验,稳定性试验,回收率试验等方面考察苯酚-硫酸法和蒽酮-硫酸法多糖测定方法。结果苯酚-硫酸法测定白术多糖含量优于蒽酮-硫酸法。结论苯酚-硫酸法测定白术多糖含量,不需要精密仪器,操作简便,稳定可靠,适合白术及其制品中多糖的常规检测,可为植物多糖含量测定方法选择提供参考。 |
| 关 键 词： | 白术粗多糖 蒽酮硫酸法 苯酚硫酸法 |
The Method Comparison of Determining Polysaccharide Content in Atractylodes macrocephala Koidz. |
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| TAN Min,QIU Xi-min. The Method Comparison of Determining Polysaccharide Content in Atractylodes macrocephala Koidz.[J]. Research and Practice on Chinese Medicines, 2012, 0(2): 73-75 | |
| Authors: | TAN Min QIU Xi-min |
| Affiliation: | 1.Changde Vocational Technical College,Changde 415000,China;2.Medical College,Hunan Normal University,Changsha 410006,China) |
| Abstract: | Objective To select a more accurate and convenient method of determining Crude Atractylodes macrocephala Koidz.Polysaccharide(CAMP) content.Methods The content was determined by two method of phenol sulfuric acid colorimetric method and anthranone sulfuric acid method.Results The comparison showed phenol-sulfuric acid method was more stable and accurate than phenol sulfuric acid colorimetric method.The recovery was higher.Conclusion So the better method to determine the content was phenol sulfuric colorimetric acid. |
| Keywords: | Atractylodes macrocephala Koidz. polysaccharide anthranone sulfuric acid method phenol sulfuric acid colorimetric method |
| 本文献已被 CNKI 维普 等数据库收录！ | |