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A GABAergic depolarizing potential in the hippocampus disclosed by the convulsant 4-aminopyridine |
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| Authors: | Massimo Avoli Paul Perreault |
| Affiliation: | 1. Institute of Radiation Physics, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland;2. ACRO, Association pour le Contrôle de La Radioactivité Dans L’Ouest, 138, Rue de L’Eglise, 14200, Hérouville St Clair, France;3. From the Goethe-University, Institute of Biophysics, Max-von-Laue-Strasse 1, D-60438, Frankfurt am Main, Germany,;4. Max Planck Institute of Biophysics, Department of Structural Biology, Max-von-Laue-Strasse 3, D-60438, Frankfurt am Main, Germany,;5. University of Regensburg, Faculty of Biology and Preclinical Medicine, Universitätsstrasse 31, D-93051, Regensburg, Germany |
| Abstract: | Intracellular recordings from hippocampal pyramidal cells in the CA1 subfield of the 'in vitro' slice in the presence of 4-aminopyridine (4-AP, 5-50 microM) revealed a long-lasting (up to 1.5 s) depolarizing potential which occurred either spontaneously or following orthodromic stimulation. This potential was: capable of blocking both direct and synaptic activation of the cell; sensitive to bath application of low concentrations of bicuculline methiodide; and associated to an extracellular current sink in the dendrites as suggested by the extracellular field potentials recorded at different levels along an axis perpendicular to the stratum pyramidale. It is concluded that the long-lasting depolarizing potential evoked by 4-AP is caused by the activation of GABA receptors localized in the dendritic region of the CA1 subfield. |
| Keywords: | 4-Aminopyridine γ -Aminobutyric acid (GABA) Hippocampus Inhibition |
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