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| MicroRNA-29c在单侧输尿管梗阻大鼠中的表达及意义 | |
| 引用本文： | 谢媚媚,章慧娣,黄慧雅,苏震,尤小寒,薛向阳. MicroRNA-29c在单侧输尿管梗阻大鼠中的表达及意义[J]. 温州医学院学报, 2012, 42(3): 205-208 |
| 作者姓名： | 谢媚媚 章慧娣 黄慧雅 苏震 尤小寒 薛向阳 |
| 作者单位： | 1. 温州医学院附属第一医院肾内科,浙江温州,325000 2. 温州医学院微生物学与免疫学教研室,浙江温州,325000 |
| 基金项目： | 国家自然科学基金资助项目，浙江省卫生高层次创新人才培养工程项目，浙江省钱江人才计划项目 |
| 摘 要： | 目的:观察microRNA-29c(miR-29c)在大鼠单侧输尿管梗阻(UUO)模型肾组织中的表达变化,探讨其是否参与肾间质纤维化及其可能机制.方法:高通量测序技术和实时荧光定量PCR检测miR-29c在UUO大鼠术后1d和3d肾脏组织中的表达情况.使用生物信息学预测miR-29c靶mRNA,对其靶mRNA进行Gene Ontology和pathway分析.结果:UUO大鼠术后1 d和术后3d手术侧肾组织miR-29c表达下调,Co1 I mRNA和FN mRNA的表达升高.预测靶mRNA经分析后获得miR-29c有统计学意义的10个靶mRNA,其中6个编码细胞外基质蛋白.结论:miR-29c在大鼠UUO模型早期的肾组织中表达下调,可能通过促进细胞外基质堆积参与肾间质纤维化的发生发展. |
| 关 键 词： | miR-29c 单侧输尿管梗阻 肾间质纤维化 大鼠 |
Expression and significance of microRNA-29c in unilateral ureteral obstruction of rat |
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| XIE Meimei , ZHANG Huidi , HUANG Huiya , SU Zhen , YOU Xiaohan , XUE Xiangyang. Expression and significance of microRNA-29c in unilateral ureteral obstruction of rat[J]. Journal of Wenzhou Medical College, 2012, 42(3): 205-208 | |
| Authors: | XIE Meimei ZHANG Huidi HUANG Huiya SU Zhen YOU Xiaohan XUE Xiangyang |
| Affiliation: | .Department of Nephrology,the First Affiliated Hospital of Wenzhou Medical College,Wenzhou,325000 |
| Abstract: | Objective: To observe the change of expression pattern of miR-29c in kidney of unilateral ureteral obstruction(UUO) rats and discuss whether miR-29c take part in renal interstitial fibrosis(RIF) and its probable mechanism.Methods: High-throughput sequencing and real-time quantitative PCR analysed the expression of miR-29c in the kidney of UUO rats,which were killed 1 and 3 days after operation.The miR-29c targets were predicted by bioinformatics and then analysed with Gene Ontology analysis and pathway analysis.Results: The expression of miR-29c decreased,while the expression of Col I mRNA and FN mRNA increased in the surgical kidney of UUO rats.Ten miR-29c targets with statistical significance were obtained after analysis of thom,6 targets coded were extracellular matrix proteins.Conclusion: The expression of miR-29c decreased in the kidney of early UUO rat model,miR-29c may participate in RIF induced by UUO through promoting extracellular matrix accumulation. |
| Keywords: | miR-29c unilateral ureteral obstruction renal interstitial fibrosis rats |
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