肿瘤相关成纤维细胞促进肺癌细胞表达PD-L1

背景与目的 肿瘤相关成纤维细胞(tumor-associated fibroblasts,TAF)是肿瘤微环境的重要组成部分,可抑制免疫细胞的功能.在肿瘤免疫中CD8+T细胞发挥重要的作用,T细胞膜表面程序性死亡因子1(programmed death factor 1,PD-1),与其配体PD-L1(programmed death factor ligand 1,PD-L1)结合对T细胞的激活起负调节作用.本研究旨在探讨TAF对肺癌细胞PD-L1表达的影响.方法 我们以肺癌细胞株H1975、H520和TAF细胞进行Transwell非接触式共培养48 h的H1975、H520细胞为实验组,单独培养的H1975、H520细胞为对照组,两组培养条件一致.倒置显微镜计数实验组和对照组H1975、H520细胞数、流式细胞仪分别检测实验组和对照组肺癌细胞PD-L1的蛋白表达率、RT-PCR分别检测实验组和对照组肺癌细胞PD-L1 mRNA的表达.结果 每100μm2细胞计数,H1975细胞实验组为(46±21)个,对照组为(16±5)个(P<0.05);H520细胞实验组为(38±10)个,对照组为(12±5)个(P<0.05).PD-L1蛋白表达率,H1975细胞实验组为(20.93%±3.54%),对照组为(12.58%±2.52%)(P<0.05);H520细胞实验组(19.26%±3.04%),对照组为(11.60%±2.65%)(P<0.05).mRNA表达水平,H1975细胞实验组为(16.45±1.25)pg/mL,对照组为(7.78±1.27)pg/mL(P<0.05);H520细胞实验组为(15.38±2.02)pg/mL,对照组为(7.20±1.58)pg/mL(P<0.05).结论 TAF促进肺癌细胞株H1975、H520的生长,增强细胞株PD-L1表达.

Tumor Associated Fibroblasts Promote PD-L1 Expression in Lung Cancer Cells

Background and objective Tumor-associated fibroblasts (TAF) is an important part of TME, which inhibits the function of immune cells. CD8+ T cells play a significant role in tumor immunity. T-cell membrane possesses a distinct type of molecule with a negative regulatory function. Upon interaction with its corresponding ligand [programmed death factor ligand 1 (PD-L1)], programmed death factor 1 (PD-1) is activated and thus inhibits the kinase activity of T cells. This study aims to explore the possible effects of TAF on PD-L1 expression in lung cancer cells. Methods Lung cancer cell lines H1975 and H520 were co-cultured with (experiment) or without TAF (control) via Transwell assay for through 48 hours under the same culture condition. H1975 and H520 cells were counted using a microscope. The protein and mRNA expression levels of PD-L1 were detected by FCM assay and PCR analysis, respectively. Results The numbers of lung cancer cells in 100μm2 for H1975 and H520 cells are (46±21) and (38±10) in the experiment group, respectively, and (16±5) and (12±5) in the control group, respectively (P<0.05). The expression levels of the PD-L1 protein in H1975 and H520 cells are (20.93%±3.54%) and (19.26%±3.04%) in the experiment group, respectively, and (12.58%±2.52%) and (11.60%±2.65%) in the control group, respectively (P<0.05). The mRNA expression levels in H1975 and H520 cells are (16.45±1.25) and (15.38±2.02) pg/mL in the experiment group, respectively, and (7.78±1.27) and (7.20±1.58) pg/mL (P<0.05) in the control group, respectively (P<0.05). Conclusion TAF promotes the growth and increases the expression of PD-L1 in H1975 and H520 cells.