| 低分子肝素分子量及其分布的测定 |
| |
| 引用本文： | 范慧红,刘金秀,徐康森. 低分子肝素分子量及其分布的测定[J]. 中国药学杂志, 1999, 0(5): 332-334 |
| |
| 作者姓名： | 范慧红 刘金秀 徐康森 |
| |
| 作者单位： | 中国药品生物制品检定所 |
| |
| 摘 要： | 目的:建立一种低分子肝素分子量及其分布测定的质量控制方法?方法:采用高效凝胶渗透色谱法(HPGPC),色谱柱为Shodex Asahipak GF-510HQ(7.6mm×300mm)或TSKG 3000SWxl(7.8mm×300mm),理论塔板数以葡萄糖峰计算分别为15 206和15 731;柱温35℃;流速0.5ml·min-1;示差折光检测器和二极管阵列检测器联用,检测波长为234nm?结果:测得两个低分子肝素样品的重均分子量和分子量分布?结论:HPGPC具有简便、快速和重现性好等特点,适合于该类药物的质量控制?
|
| 关 键 词： | 低分子肝素 高效凝胶渗透色谱法 分子量 分子量分布 |
| 收稿时间： | 1998-12-10; |
Measurement of molecular weight and molecular weight distribution of low molecular weight heparin |
| |
| Fan Huihong. Measurement of molecular weight and molecular weight distribution of low molecular weight heparin[J]. Chinese Pharmaceutical Journal, 1999, 0(5): 332-334 |
| |
| Authors: | Fan Huihong |
| |
| Affiliation: | (Fan HH)，Liu Jinxiu(Liu JX)，Xu Kangsen(Xu KS |
| |
| Abstract: | OBJECTIVE: To develop a method for the quality control of low molecular weight heparin.METHODS:Molecular weight and molecular weight distribution of LMWH was determined by high performance gel permeation chromatography(HPGPC)on Shodex Asahipak GF-510HQ column or TSK G3000SWxl column at 35℃ with 2.84%(pH5.0)sodium sulfate as the mobile phase,It was detecled by RI and UV detector at 234nm.The flow rate of the mobile phase was 0.5ml·min-1.RESULTS:The molecular weight and molecular weight distribution of two LMWH samples were obtained. RSD was less than 1%.CONCLUSION:The method is simple with good reproducibility(RSD<1%)and practicible for the control of the medicine. |
| |
| Keywords: | low molecular weight heparin HPGPC molecular weight molecular weight distribution |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国药学杂志》浏览原始摘要信息 |
|
点击此处可从《中国药学杂志》下载免费的PDF全文 |
