牛磺酸对糖尿病大鼠膈肌酶活性的影响

目的: 观察牛磺酸对糖尿病(diabetes mellitus,DM)大鼠膈肌损伤的保护作用,探讨其作用机制。方法: SD大鼠24只,随机分为正常对照组(NC)、糖尿病组(DM)、牛磺酸治疗组(Tau)。腹腔注射链脲菌素50mg/kg制备DM大鼠模型。NC组和DM组予自来水,Tau组予1%牛磺酸饮水,4周后测大鼠体重、血糖,检测膈肌组织中的超氧化物歧化酶(SOD)、一氧化氮合酶(NOS)活性和丙二醛(MDA)、NO含量的变化;电镜下观察膈肌组织超微结构的改变。结果: 与NC组相比,DM组大鼠膈肌组织中SOD、NOS活性、NO含量显著降低(P<0.01),MDA含量显著增加(P<0.01);电镜下,肌小节和线粒体形态改变。与DM组相比,Tau组SOD、NOS活性、NO含量增加(P<0.05和P<0.01),血糖和MDA含量降低(P<0.01);电镜下,超微结构损伤减轻。结论: 牛磺酸可提高DM大鼠膈肌组织中SOD、NOS活性和NO含量,降低血糖浓度、MDA含量,能保护DM膈肌的损伤。

Effects of taurine on activity of enzymes in tissues of diaphragm in diabetic rats

Objective:To study the effects of taurine on diaphragm injury in diabetic rats.Methods:Twenty-four SD rats were randomly divided into normal control(NC) group,diabetic(DM) group and taurine treatment(Tau) group.DM group and Tau group rats were given streptozocin(STZ) 50 mg/kg intraperitoneal injection.NC group and DM group rats were treated with watar,Tau group rats were treated with 1% taurine in drinking watar.After 4 weeks,the body weight(BW) and level of blood glucose(BG) were measured.Superoxide dismutase(SOD),nitric oxide synthase(NOS) activities and malondialdehyde(MDA),NO contents in diaphragm tissues were also measured.The ultrastructure of diaphragm were intvestigated by electron microscope.Results:SOD,NOS activities,NO contents were significantly lower(P<0.01),BG and MDA content were significantly higher in DM group(P<0.01).Compare with DM group,SOD,NOS activities and NO contents were higher(P<0.05 and P<0.01),BG and MDA content were lower in Tau group(P<0.01).The ultrastructure under electron microscope indicated that in DM group diaphragm fibres fracture,mitochondrions swelling and degeneration.The above changes were inhibited by treatment of taurine.Conclusions:Taurine can increase SOD,NOS activities,NO contents and decrease MDA conten in diabetic diaphragm.