BD926对骨髓源性未成熟树突状细胞增殖及吞噬作用的影响

目的 研究苯并噻唑类衍生物BD926对小鼠骨髓源性未成熟树突状细胞(immature dendritic cells, imDCs)增殖及吞噬作用的影响. 方法 分离小鼠骨髓源性单核细胞诱导imDCs,借助PE/Annexin V凋亡检测试剂盒、CFSE细胞增殖试剂盒以及FITC-葡聚糖,应用流式细胞术检测BD926对imDCs在粒细胞-巨噬细胞集落刺激因子(granulocyte macrophage colony stimulating factor,GM-CSF)刺激下的细胞存活、细胞增殖以及吞噬作用的影响.结果 与PBS组相比,25 、5 、1 μM 的BD926处理imDCs后,其细胞增殖水平降低,差异有统计学意义(P<0.05);高、中、低3个剂量组BD926处理后,imDCs与PBS组相比,细胞存活差异无统计学意义 (P>0.05);高、中、低3个剂量组BD926处理后,imDCs与PBS组相比,吞噬作用差异无统计学意义 (P>0.05).结论 BD926在体外可明显抑制GM-CSF刺激的imDCs增殖作用,对imDCs的细胞存活和吞噬作用则无明显影响.

The Effect of BD926 on the Proliferation and Phagocytosis of Murine Bone Marrow-Derived Immature Dendritic Cells

Objective To study the effect of the Benzothiazole derivative BD926 on the proliferation and phagocytosis of murine bone marrow-derived immature dendritic cells (imDCs).Methods ImDCs were isolated and induced from murine bone marrow-derived mononuclear cells.The flow cytometry (FCM) was used to detect the effect of BD926 on the cell survival, proliferation and phagocytosis of imDCs upon the stimulation of GM-CSF with the help of PE/Annexin V Apoptosis Detection Kit, CellTrace CFSE Cell Proliferation Kit and FITC-dextran.Results Compared with the PBS group, the cell proliferation of imDCs treated by BD926 of 25μM, 5μM and 1μM respectively were significantly reduced in a concentration-dependent manner (P<0.05), while the survival and phagocytosis of imDCs treated by BD926 of the high, medium and low dosage respectively were not statistically different from those of imDCs in the PBS group (P>0.05).Conclusion The Benzothiazole derivative BD926 can suppress the proliferation of imDCs stimulated by GM-CSF in vitro, and it has no obvious effect on the survival and phagocytosis of imDCs.