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| RGD肽修饰的纯钛种植材料表面成骨细胞黏附增殖能力 | |
| 引用本文： | 陈奕帆,黄元瑾,宋光保,万乾炳,王剑,杨晓喻,巢永烈. RGD肽修饰的纯钛种植材料表面成骨细胞黏附增殖能力[J]. 实用口腔医学杂志, 2010, 26(1). DOI: 10.3969/j.issn.1001-3733.2010.01.01 |
| 作者姓名： | 陈奕帆 黄元瑾 宋光保 万乾炳 王剑 杨晓喻 巢永烈 |
| 作者单位： | 1. 广东省口腔医院南方医科大学附属口腔医院修复科,广州,510280 2. 成都四川大学口腔疾病国家重点实验室 |
| 基金项目： | 国家自然科学基金资助项目，广东省医学科学技术研究基金资助项目 |
| 摘 要： | 目的:评价精氨酸-甘氨酸-天冬氨酸(RGD)肽修饰纯钛表面对细胞早期黏附增殖影响. 方法:利用分子自组装技术氨基化纯钛表面,化学接枝GYRGDS肽,计算不同时点材料表面黏附细胞数;不同浓度RGD肽液预处理接种前细胞,计算材料表面细胞黏附率;MTT法和扫描电镜分别检测材料表面细胞的增殖、生长.结果:RGD肽修饰的纯钛表面细胞早期黏附率、增殖活性和生长情况在各时点均优于未修饰组,高浓度肽液预处理对RGD肽修饰组表面细胞有较强黏附抑制作用.结论:采用分子自组装技术可将活性RGD肽稳定的化学接枝于纯钛表面,修饰后材料的细胞早期黏附增殖等生物学行为有明显改善. |
| 关 键 词： | 纯钛 RGD肽 成骨细胞 |
A study of the osteoblasts adhesion,growth and proliferation on the surface of pure titanium immobilized by RGD peptide |
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| CHEN Yifan,HUANG Yuanjin,SONG Guangbao,WAN Qianbing,WANG Jian,YANG Xiaoyu,CHAO Yonglie. A study of the osteoblasts adhesion,growth and proliferation on the surface of pure titanium immobilized by RGD peptide[J]. Journal of Practical Stomatology, 2010, 26(1). DOI: 10.3969/j.issn.1001-3733.2010.01.01 | |
| Authors: | CHEN Yifan HUANG Yuanjin SONG Guangbao WAN Qianbing WANG Jian YANG Xiaoyu CHAO Yonglie |
| Affiliation: | CHEN Yifan1,HUANG Yuanjin1,SONG Guangbao1,WAN Qianbing2,WANG Jian2,YANG Xiaoyu1,CHAO Yonglie2.1.510280 Guangzhou,Department of Prosthodontics,Guangdong Provincial Stomatological Hospital,Southern Medical University,China,2.State Key Laboratory of Oral Diseases,Sichuan University,Chengdu |
| Abstract: | Objective:To evaluate the effect of pure titanium modified by bioadhesive RGD peptide on the early attachment,growth and proliferation of osteoblasts.Methods:The titanium samples were hydroxylated by alkali/hot water aging and sol-gel layer-by-layer deposition technique.Afterwards,the terminal-NH2 group was introduced to the titanium surface by organosilane APTMS self-assembled monolayers and the functional group-NH2 was further reacted with EDC/NHS by which RGD peptides was covalently immobilized to titani... |
| Keywords: | Titanium RGD peptide Osteoblasts |
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