毛细管区带电泳法测定麻黄中麻黄碱和伪麻黄碱的含量

目的建立测定麻黄中麻黄碱和伪麻黄碱含量的毛细管区带电泳（CZE）法。方法以200 mmol.L-1硼砂-500 mmol.L-1硼酸溶液（1∶1,v/v）含2 mg.mL-1庚烷磺酸钠和0.4%（v/v）乙腈为背景电解质（BGE）,石英毛细管（75 cm×75μm）,有效分离长度60 cm,运行电压15 kV,紫外检测波长210 nm,重力进样20 s（高度10 cm）,以苯甲醇作内标,对麻黄中的麻黄碱和伪麻黄碱进行定量分析,以两者含量为参量对10批麻黄进行系统聚类分析并进行质量评价。结果麻黄碱和伪麻黄碱的相对峰面积（Ai/Ar）与各自的质量浓度C（mg.mL-1）呈良好的线性关系,两者的平均回收率分别为100.4%和100.9%。结论该法准确可靠,操作便捷,可用于麻黄中麻黄碱和伪麻黄碱的含量测定。

Determination of ephedrine and pseudoephedrine in Herba Ephedra by capillary zone electrophoresis

Objective To develop a method for the determination of ephedrine and pseudoephedrine in Herba Ephedra by capillary zone electrophoresis.Methods Separation was done in an uncoated fused silica capillary（75 cm×75 μm,effective length 60 cm）with a 200 mmol·L-1 sodium borate-500 mmol·L-1 boric acid（1∶1,v/v）containing 2 mg·mL-1 sodium heptanesulfonate and 0.4%（v/v） acetonitrile under 15 kV.The detection wavelength was 210 nm and the benzyl alcohol was the internal standard.Ten batches of Herba Ephedrae were identified with hierarchical clustering analysis using the contents of ephedrine and pseudoephedrine as variables.Results There was good linear relation between the concentration and the relative peak area of ephedrine and pseudoephedrine.The average recovery of ephedrine and pseudoephedrine was 100.4% and 100.9%,respectively.Conclusion The method is rapid,simple and accurate,and it can be used to determine ephedrine and pseudoephedrine.