An HLA-A*02:01-B*13:01-DRB1*14:01:03 haplotype conserved in Taiwanese and a possible close relationship between DRB1*14:01:03 and DRB1*14:54期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

*An HLA-A02:01-B13:01-DRB114:01:03 haplotype conserved in Taiwanese and a possible close relationship between DRB114:01:03 and DRB114:54**

Authors:	Yang K L Lee S K Chu C C Lin C C Jiang S Chiu H M Lin S Chen Y C Chen M J Jian Y F Yang C F Yang S Y Shyr M J Lin C L Lin P Y

Affiliation:	Laboratory of Immunogenetics and Cord Blood Bank, Buddhist Tzu Chi Stem Cells Centre and Marrow Donor Registry, Buddhist Tzu Chi General Hospital, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan. edward@tzuchi.com.tw

Abstract:	We here report sequence confirmation and analysis of the variant HLA-DRB114:01:03 on three voluntary bone marrow donors and the conserved haplotype carrying DRB114:01:03 allele in Taiwanese population. In exon 2, the DNA sequence of DRB114:01:03 is identical to HLA-DRB114:01:01 except a silent nucleotide substitution at position 192. However, sequence specific primer (SSP) reaction pattern of DRB114:01:03 matched with the pattern of DRB114:54 instead of DRB114:01:01, 14:01:02 or 14:01:03. In exon 3, at position 421, DRB114:01:03 has an identical nucleotide as DRB114:54 but differs from DRB114:01:01. We think the discrepancy of the allele assignment by SSP typing protocol and by sequence-specific oligonucleotide probe (SSO) and sequence-based typing methods should be addressed. We assume DRB114:54 is probably the parental allele for DRB114:01:03.

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