滋阴通下药对硫代乙酰胺致肝损伤模型大鼠炎性细胞因子的影响

目的观察滋阴通下药及通下药对硫代乙酰胺（TAA）所致内毒素血症模型大鼠炎性细胞因子水平的影响。方法建立TAA所致大鼠肝损伤内毒素血症模型，以滋阴通下药加味小承气汤、通下药大承气汤、阳性对照药乳果糖进行干预，采用血液生化及ELISA法检测，观察其对模型大鼠内毒素、肿瘤坏死因子（TNF-α）、丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）水平的影响。结果滋阴通下药组、通下药组、乳果糖组肝损伤大鼠血清中内毒素、TNF-α、ALT、AST水平明显低于模型组（P〈0．01）；滋阴通下药组与通下药组比较内毒素、TNF-α、ALT、AST水平差异有统计学意义（P〈0．05）。结论滋阴通下药及通下药均为治疗肝病内毒素血症的有效方药，滋阴通下药疗效优于单纯通下药。

Effect of yin-nourshing purgative medicine on inflammatory cytokines in thioacetamide-induced liver injury rats

Objective To observe the effect of yin-nourshing purgative medicine and purgative medicine on inflammatory cytokines level in thioacetamide （TAA）-induced endotoxemia rats. Methods The TAA-induced endotoxemia model was established and intervened by yin-nourshing purgative medicine （Jiawei Xiaochengqi Decoction）, purgative medicine （Dachengqi Decoction） and positive control drug （lactulose）. With blood biochemistry and ELISA method, the level of endotoxin, tumor necrosis factor （TNF- -α ）, alanine aminotransferase （ALT） and aspartate aminotransferase （AST） was detected. Results The level of endotoxin, TNF- -α, ALT and AST in the blood of the liver injury rats in the yin-nourshing purgative medicine, purgative medicine and lactulose groups was significantly lower than that in the model group （P 〈0.01）; When comparing the yin-nourshing purgative medicine group with the purgative medicine group, the level showed significant difference （P 〈0.05）. Conclusion The yin-nourshing purgative medicine and purgative medicine are effective in treating endotoxemia of liver disease and the efficacy of the yin-nourshing purgative medicine is superior to that of the purgative medicine.