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| 蛞蝓有效提取物对人肺癌A549细胞增殖的影响 | |
| 引用本文： | 王娟,刘丽华,郑兴. 蛞蝓有效提取物对人肺癌A549细胞增殖的影响[J]. 湖南师范大学学报(医学版), 2008, 5(4) |
| 作者姓名： | 王娟 刘丽华 郑兴 |
| 作者单位： | 南华大学药物药理研究所,湖南,衡阳,421001;南华大学药物药理研究所,湖南,衡阳,421001;南华大学药物药理研究所,湖南,衡阳,421001 |
| 摘 要： | 目的:筛选蛞蝓有效提取物,并观察蛞蝓有效提取物EL-3对人肺癌A549细胞增殖的抑制作用。方法:体外培养人肺癌A549细胞和中国仓鼠肺上皮CHL细胞;MTT比色法测定细胞活性;软琼脂集落形成法检测细胞锚定非依赖性生长能力。结果:以不同提取方式获得的6种蛞蝓提取物对人肺癌A549细胞增殖活性具有抑制作用,其中以EL-3抑制作用最强,其IC50值为9.4μg/mL,可作为蛞蝓有效提取物;EL-3选择性抑制A549细胞活性,其选择指数是43.6;EL-3能有效抑制A549细胞锚定非依赖性生长,呈浓度依赖性。结论:EL-3具有选择性抑制A549细胞增殖作用。 |
| 关 键 词： | 肺癌 蛞蝓 蛞蝓有效提取物 治疗作用 |
Effect of the Effective Extraction of Limax on Proliferation of Human Lung Carcinoma A549 Cell Line |
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| WANG Juan,LIU Li-hua,ZHENG Xing. Effect of the Effective Extraction of Limax on Proliferation of Human Lung Carcinoma A549 Cell Line[J]. Journal of Hunan Normal University(Medical Science), 2008, 5(4) | |
| Authors: | WANG Juan LIU Li-hua ZHENG Xing |
| Affiliation: | WANG Juan; LIU Li-hua; ZHENG Xing(Institute of Pharmacy and Pharmacology; University of South China; Hunan; Hengyang 421001; China); |
| Abstract: | Objective To screen the effective extraction of Agriolimax agrestis(Limax) to against human lung carcinoma and to observe the proliferation inhibitory effect of EL-3.Methods Human lung carcinoma A549 cells and Chinese hamster lung epithelial CHL cells were incubated in vitro.The cell viability was examined by MTT assay.The non-anchorage dependent growth ability was determined by colony formation in soft agar method.Results Six kinds of extraction from Limax prepared by different ways inhibited the cell viab... |
| Keywords: | lung carcinoma Limax effective extraction of Limax therapeutic action |
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