原发性干燥综合征差异基因及潜在治疗药物的生物信息分析

目的 利用基因表达谱筛选与原发性干燥综合征（pSS）相关基因和潜在小分子治疗药物。方法 在GEO数据库获取并分析3个pSS相关基因表达谱，获得差异表达基因，并对差异基因进行GO与KEGG富集分析，通过蛋白互作网络（PPI）筛选pSS的hub基因并验证。最后，利用Cmap数据库预测pSS的潜在治疗药物，并结合药物靶点和药物途径探讨潜在药物在pSS治疗中的作用。结果 共鉴定出90个pSS相关差异基因，其中79个上调基因， 11个下调基因，它们主要参与NOD样受体信号通路、甲型流感和细胞因子 细胞因子受体相互作用通路。此外，通过筛选、验证，鉴定出19个hub基因（STAT1、MX1、ISG15、IFIH1、GBP1、IFIT1、XAF1、RSAD2、IFIT2、SAMD9L、TRIM22、IFIT3、IFI44L、HERC5、IFIT5、IFI44、IFI6、RTP4和ISG20），多个是干扰素诱导基因。干扰素可能在pSS发病中起重要作用。最后，通过CMAP数据库筛选出氟替卡松、氯唑西林等15种候选药物，并对其作用通路与靶点分析。结论 本研究通过对pSS hub基因和候选药物的筛选，可以进一步了解pSS的发病机制，为pSS的进一步研究提供线索。

Bioinformatics analysis of differential genes and potential therapeutic drugs in primary Sjgren's syndrome

Objective To screen genes associated with primary Sjgren's syndrome (PSS) and potential small molecule therapeutic drugs by gene expression profiles. Methods The expression profiles of three PSS related genes were obtained and analyzed in GEO databaseto obtain differentially expressed genes.Furthermore,GO and KEGG enrichmentanalysiswere carried out on the differential genes, andthehub genes of PSS were screened and verified by PPI. Finally, CMAP database was used to predict the potential therapeutic drugs of PSS, and the role of potential drugs in PSS treatment was discussed in combination with drug targets and drug pathways. Results A total of 90 PSS related differential genes were identified, including 79 up regulated genes and 11 down regulated genes, which were mainly involved in NOD like receptor signaling pathway, Influenza A and cytokine cytokine receptor interaction pathway. In addition, through screening and verification, 19hub genes (STAT1, MX1, ISG15, IFIH1, GBP1, IFIT1, XAF1, RSAD2, IFIT2, SAMD9L, TRIM22, IFIT3, IFI44L, HERC5, IFIT5, IFI44, IFI6, RTP4 and ISG20) were identified, most of which were interferon induced genes. Interferon may play an important role in the pathogenesis of PSS. Finally, 15 candidate drugs such as fluticasone and cloxacillin were selected through CMAP database, and their action pathways and targets were analyzed.Conclusion In this study,through the screening of PSS hub genes and candidate drugs,we can further getanin sight into the pathogenesis of PSS and the studyprovides clues for thefurther study of PSS.