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| 抗体包被免疫磁珠分离CD4+T淋巴细胞亚群的方法学研究和初步应用 | |
| 引用本文： | 王岚,金焕英,王萃,吴玲玲,戴铁颖,樊丹君,郑筱叶. 抗体包被免疫磁珠分离CD4+T淋巴细胞亚群的方法学研究和初步应用[J]. 中国感染控制杂志, 2005, 4(2): 112-114 |
| 作者姓名： | 王岚 金焕英 王萃 吴玲玲 戴铁颖 樊丹君 郑筱叶 |
| 作者单位： | 浙江大学医学院传染病研究实验室,浙江,杭州,310000 |
| 摘 要： | 目的 应用抗体包被免疫磁珠分离CD4 +T淋巴细胞亚群,并检测其效果。方法 运用直接包被法制备免疫磁珠,并以流式细胞仪分析。结果 CD4 +T淋巴细胞亚群分离率>95 %。结论 用直接包被法制备的免疫磁珠具有较好的分离CD4 +T淋巴细胞亚群效果。 |
| 关 键 词： | 免疫磁珠 CD4＋T淋巴细胞亚群 直接包被法 方法 |
| 文章编号： | 1671-9638(2005)02-0112-03 |
| 修稿时间： | 2004-08-13 |
Method evaluation and preliminary application of antibody-coating immunomagnetic beads to isolate CD4 + T lymphocyte subpopulation |
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| WANG Lan,JIN Huan-ying,WANG Cui,WU Ling-ling,DAI Tie-ying,FAN Dan-jun,ZHENG Xiao-ye. Method evaluation and preliminary application of antibody-coating immunomagnetic beads to isolate CD4 + T lymphocyte subpopulation[J]. Chinese Journal of Infection Control, 2005, 4(2): 112-114 | |
| Authors: | WANG Lan JIN Huan-ying WANG Cui WU Ling-ling DAI Tie-ying FAN Dan-jun ZHENG Xiao-ye |
| Abstract: | Objective To isolate CD4+ T lymphocyte subpopulation by antibody-coating immunomagnetic beads and evaluate the efficacy of detective methods. Methods Immunomagnetic beads were prepared by direct antibody coating and analysed by flow cytometry. Results The isolation rate of CD4+ T lymphocyte subpopulation was above 95%. Conclusion Direct antibody coating of immunomagnetic beads can isolate CD4+ T lymphocyte subpopulation effectively. |
| Keywords: | immunomagnetic beads CD4+ T lymphocyte subpopulation direct coating methods |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |