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| 印迹基因H_(19)在少弱精子症中印迹状态的检测 | |
| 引用本文： | 叶静,龙霞,张芳婷,卢丽华,房家智. 印迹基因H_(19)在少弱精子症中印迹状态的检测[J]. 陕西医学杂志, 2008, 37(1): 47-49 |
| 作者姓名： | 叶静 龙霞 张芳婷 卢丽华 房家智 |
| 作者单位： | 1. 北京大学深圳医院中心实验室,深圳,518036 2. 北京大学深圳医院检验科 |
| 摘 要： | 目的:通过研究印迹基因H19在少弱精子症中的印迹状态,探讨H19与精子发生障碍的相关性。方法:采用聚合酶链式反应与逆转录-聚合酶链式反应,结合限制性片段长度多态性分析法(RFLP),对36例异常精液和15例正常精液标本进行H19印迹状态检测。结果:15例正常精液没有发现印迹丢失,36例异常精液检测出3例印迹丢失,印迹丢失率为8.3%。结论:H19印迹丢失与精子发生障碍有关,可能影响精子的数量。 |
| 关 键 词： | 少精液症/诊断 印迹法,DNA 基因组印迹 |
| 收稿时间： | 2007-08-22 |
| 修稿时间： | 2007-08-22 |
The imprinting status of imprinting gene H_(19) detected in oligospermia and asthenospermia |
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| Ye Jing Long Xia Zhang Fangting et al Central Laboratory,Shenzhen Hospital of Peking University. The imprinting status of imprinting gene H_(19) detected in oligospermia and asthenospermia[J]. Shaanxi Medical Journal, 2008, 37(1): 47-49 | |
| Authors: | Ye Jing Long Xia Zhang Fangting et al Central Laboratory Shenzhen Hospital of Peking University |
| Affiliation: | Shenzhen 518036 |
| Abstract: | Objective: To study the imprinting status of H19 in oligospermia and asthenospermia and to investigate the correlation between H19 and spermatogenesiss disturbance. Methods: PCR-RFLP and RT-PCR-RFLP were used to detect the imprinting status of H19 in the abnormal(36 cases) and normal(15 cases) semen. Results: There was no loss of imprintion in the normal semen. Loss occured of imprintion in 3 cases of the abnormal semen. The rate was 8.3%. Conclusion: The loss of imprinting of H19 is related to spermatogenesiss disturbance. It perhaps affects the quantity of semen. |
| Keywords: | Oligospermia/diagnosis Blotting Southern Gernomic imprinting |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |