麻风康复者外周血Treg细胞及IL-10水平检测

目的：通过检测麻风康复者外周血中CD4+CD25+Foxp3+Treg细胞百分比、IL-10的表达水平,探讨麻风康复者的细胞免疫水平。方法：采用流式细胞技术检测25例麻风康复者和20名健康者对照的外周血中CD4+CD25+Foxp3+Treg细胞的水平变化,ELISA法检测血清中IL-10水平,并进行相关性分析。 结果：麻风康复者组外周血CD4+CD25+Foxp3+Treg细胞占CD4+CD25+Treg淋巴细胞的比率和血清IL-10浓度分别为(7.45±1.43)%和(13.45±3.61)pg/mL,均高于正常对照组的(5.89±1.21)%和(9.23±1.51)pg/mL（P＜0.01）。麻风康复者及正常对照外周血CD4+CD25+Foxp3+Treg细胞水平与血清中IL-10含量呈正相关（P＜0.05）。结论：麻风康复者外周血中CD4+CD25+Foxp3+Treg细胞与血清中IL-10高于正常对照,麻风康复者可能存在细胞免疫失调状态。

Detection of Treg Cells and IL-10 in the Peripheral Blood of the Cured Patients with Leprosy

Objective: To detect the level of CD4+CD25+Foxp3+Treg cells and interleulin-10 (IL-10) in the peripheral blood from the cured patients with leprosy in order to explore the mimmune status of them. Methods: The levels of CD4+CD25+Foxp3+Treg in T lymphocyte subgroups and IL-10 in peripheral blood were detected by flow cytometry and ELISA respectilvely in 25 cured patients with leprosy and 20 healthy controls. Results: The level  CD4+CD25+Foxp3+Treg in T lymphocytes and the level of IL-10 in the patients were (7.45±1.43)% and (13.45±3.61) pg/mL, which were higher than those in controls (5.89±1.21)% and (9.23±1.51) pg/mL, with significant differences (P<0.01). There was a significant positive correlation between  CD4+CD25+Treg cells and IL-10 in the cured patients and healthy controls. Conclusion: The level of CD4+CD25+Foxp3+Treg cells and IL-10 in blood serum in the cured patients with leprosy were higher than those in healthy controls, which indicated the cured patients with leprosy may have Immune imbalance.