| 外周致密纤维1在人精子中的表达差异及其意义 |
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| 引用本文： | 陈静,王勇,许祥,余州,桂耀庭,蔡志明.外周致密纤维1在人精子中的表达差异及其意义[J].中华男科学杂志,2009(10):891-894. |
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| 作者姓名： | 陈静 王勇 许祥 余州 桂耀庭 蔡志明 |
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| 作者单位： | 北京大学深圳医院男性生殖与遗传重点实验室;汕头大学医学院生理系; |
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| 基金项目： | 国家自然科学基金(30770810);;广东省科技计划项目(2007B060401061);;深圳市科技计划项目(200903078)~~ |
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| 摘 要： | 目的:比较外周致密纤维1(ODF1)在健康男性和弱精子症患者精子中的表达差异。方法:根据WHO标准,收集正常男性和弱精子症患者的精液标本各20份,Percoll非连续梯度离心法分离精子,收集95%Percoll以下和57%与76%Percoll层之间的精子,以排除生精细胞和白细胞的污染;采用逆转录-多聚酶链式反应(RT-PCR)和Western印迹方法,从mRNA和蛋白水平检测ODF1的表达。结果:RT-PCR结果表明,与正常男性组相比,ODF1在弱精子症患者精子中的mRNA表达水平显著降低(2.79±0.28vs1.35±0.25,P<0.05);免疫印迹与RT-PCR结果一致,与正常男性组相比,弱精子症患者精子中的ODF1蛋白的表达亦显著降低(3.64±0.34vs1.44±0.26,P<0.05)。结论:ODF1在弱精子症患者精子中的表达显著降低,提示其可能与精子活动力低下有关。
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| 关 键 词： | 精子活动率 外周致密纤维1 弱精子症 基因表达 |
Differential Expression of ODF1 in Human Ejaculated Spermatozoa and Its Clinical Significance |
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| CHEN Jing,WANG Yong,XU Xiang,YU Zhou,GUI Yao-ting,CAI Zhi-ming.Key Laboratory of Male Reproductive , Genetic Medicine,Shenzhen Hospital of Peking University,Shenzhen,Guangdong ,China.Differential Expression of ODF1 in Human Ejaculated Spermatozoa and Its Clinical Significance[J].National Journal of Andrology,2009(10):891-894. |
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| Authors: | CHEN Jing WANG Yong XU Xiang YU Zhou GUI Yao-ting CAI Zhi-mingKey Laboratory of Male Reproductive Genetic Medicine Shenzhen Hospital of Peking University Shenzhen Guangdong China |
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| Institution: | CHEN Jing1,WANG Yong1,2,XU Xiang1,YU Zhou1,GUI Yao-ting1,CAI Zhi-ming11.Key Laboratory of Male Reproductive , Genetic Medicine,Shenzhen Hospital of Peking University,Shenzhen,Guangdong 518036,China,2.Department of Physiology,Medical College of Shantou University,Shantou,Guangdong 515041 |
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| Abstract: | Objective: To compare the expressions of ODF1(outer dense fiber of the sperm tail 1) in ejaculated spermatozoa from normozoospermic and asthenozoospermic men with low sperm motility.Methods: Semen analyses were performed on the semen samples obtained from normozoospermic(n=20) and asthenozoospermic(n=20) volunteers according to the WHO criteria.To rule out the contamination of germ cells and leucocytes,the human ejaculated spermatozoa were purified by a discontinuous Percoll density gradient centrifugation.... |
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| Keywords: | sperm motility outer dense fiber of the sperm tail 1 asthenozoospermia gene expression |
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