姜黄素对大鼠心肌缺血再灌注损伤的保护作用

目的:观察姜黄素对大鼠心肌缺血再灌注损伤的保护作用.方法:结扎大鼠冠状动脉左前降支,使心肌缺血60 min,再灌注60 min,制成急性心肌缺血再灌注损伤模型,缺血前5 min,舌下静脉推注姜黄素.再灌结束时测量心肌梗死面积,测定血清肌酸磷酸激酶(CK),乳酸脱氢酶(LDH),心肌超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶活性(GSH-Px)及心肌丙二醛(MDA)、游离脂肪酸(FFA)含量.结果:姜黄素(20,40 mg/kg)能减少心肌梗死面积,降低血清CK、LDH活性,提高心肌SOD、GSH-Px活性,减少心肌MDA、FFA含量.结论:姜黄素对心肌缺血再灌注损伤的保护作用,与减少脂质过氧化物和增加内源性抗自由基物质的产生,纠正心肌脂肪酸代谢紊乱有关.

Protective Effect of Curcumin on Myocardial Ischemia Reperfusion Injury in Rats

OBJECTIVE: To study the protective effect of curcumin on myocardial reperfusion injury in rats. METHODS: Myocardial ischemia reperfusion injury model was established by occluding the left anterior descending branch of coronary arter for 60 min and removing the ligation later to reperfuse for 60 min in vivo. Different dose of curcumin were administered by intravenous injection 5 min before the onset of ischemia. The changes of myocardial infarct sizes (MIS), the serum creatine phosphokinase (CK) and lactate dehydrogenase (LDH), the myocardial malondialdehyed (MDA) and free fatty acid (FFA) content, the myocardial super oxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity were measured. RESULTS: Curcumin (20, 40 mg/kg) reduced the myocardial infarct sizes, the serum CK and LDH activity. The myocardial MDA and FFA content all declined. SOD and GSH-Px activity were increased markedly. CONCLUSION: Curcumin exerts protective effects on myocardial ischemia reperfusion injury, which may be due to its function of inhibition of lipid peroxidation, augmentation of endogenous antioxidants and improving myocardial metabolism.