谷胱甘肽对锰染毒大鼠抗氧化能力的影响

目的研究谷胱甘肽(GSH)对锰染毒大鼠抗氧化能力的影响。方法将48只清洁级健康雄性SD大鼠随机分为6组,即空白对照组(生理盐水)、拮抗组(1 mmol/kg GSH)、低剂量染毒组(15 mg/kg MnCl2.4H2O)、高剂量染毒组(30mg/kgMnCl2.4H2O)、低剂量染毒拮抗组(15 mg/kgMnCl2.4H2O和1 mmol/kgGSH)和高剂量染毒拮抗组(30 mg/kg MnCl2.4H2O和1 mmol/kgGSH),每组8只。采用化学比色法检测血清和睾丸组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)的活力,并对睾丸组织进行病理学检查。结果与空白对照组比较,高剂量染毒组血清和睾丸组织SOD、CAT及GSH-Px活力均下降(P<0.01);低剂量染毒组血清SOD、CAT、GSH-Px活力和睾丸SOD活力均下降(P<0.05,P<0.01);高剂量染毒拮抗组血清和睾丸组织SOD和CAT活力明显下降(P<0.01)。与相同剂量的单纯锰染毒组比较,低剂量染毒拮抗组血清和睾丸组织SOD、CAT、GSH-Px活力均恢复至对照组水平;高剂量染毒拮抗组血清SOD、CAT、GSH-Px活力均明显上升(P<0.01),睾丸组织SOD和GSH-Px活力明显上升(P<0.01)。病理检查可见低剂量染毒组和高剂量染毒组睾丸生精小管呈现不同程度的变性,生精上皮变薄,管腔内精子较少或缺如。低剂量染毒拮抗组睾丸病理学的改变基本恢复正常;高剂量染毒拮抗组睾丸仍然可见变性的生精小管,管腔内精子较少见。结论GSH可拮抗锰染毒大鼠抗氧化能力的降低,对机体起一定的保护作用。

Antagonism of Glutathione to Antioxidative Capacity Decrease Induced by Manganese Exposure in Rats

Objective To study the antagonism of glutathione(GSH) to antioxidative capacity decrease induced by manganese exposure in rats.Methods 48 healthy male SD rats were randomly divided into six groups: the blank control,GSH control,MnCl2.4H2O(15 mg/kgand 30 mg/kg),15 mg/kgMnCl2.4H2O 1 mmol/kgGSH and 30 mg/kg MnCl2.4H2O 1 mmol/kg GSH,the treatment was conducted through peritoneal injection.The activity of superoxide dismutase(SOD),catalase(CAT) and glutathione peroxidase(GSH-Px)in the serumand the testis were determined bycolorimetric analysis.Results Compared with the blank control group,the activity of SOD,CAT and GSH-Px in the serum and testis in 30 mg/kg groups significantly decreased(P< 0.01).The activity of SOD and CAT in the serum and testis in 30 mg/kg GSH group decreased significantly(P<0.01).Compared with the single manganese treated groups,all antioxidase activities in the serum and testis in 15 mg/kg GSH group increased significantly(P<0.01).All antioxidase activities in the serum increased significantly(P<0.01) and SOD and GSH-Px in the testis increased significantly(P<0.01)in 30 mg/kg GSH group.Pathological examination showed the different degree of denaturation in seminiferous tubule and the decrease of spermatogenic epithelium and spermiogenesis in 15 mg/kg and 30 mg/kg groups.The testicular lesions returned to the normal level in histopathology in 15 mg/kg GSH group.It was shown that the seminiferous tubule was denatured and the spermiogenesis decreased in 30 mg/kg GSH group.Conclusion GSH can antagonize antioxidative capacitydecrease induced bymanganese exposure in rats.