H2O2浸渍处理对水牛角胎力学特性的影响

背景：前期实验数据证明，水牛角胎的形态结构、理化参数、生物性质、力学性能等方面具有良好的骨科生物材料的特征。目的：拟观察脱蛋白处理水牛角胎后，其抗原性和生物力学强度与经30％H2O2处理的时间关系。设计、时间及地点：测量实验，于2000-02／03在昆明理工大学力学试验室完成。材料：水牛来源于云南省泸西县屠宰场，取水牛角胎制成公称尺寸试件。方法：试件经热蒸馏水反复冲洗后，用氯仿-甲醇1：1浸渍48h，再用体积分数为0.3的H2O2浸渍，实验组分别处理24，48h，另设未经任何处理试件为对照组。主要观察指标：电测试法测定弹性模量E；直接加压法测其抗压强度。结果：各组间弹性模量相比，差异均无显著性意义（P〉0．05）。对照组的抗压强度明显高于实验组（P〈0．01），24h实验组与48h实验组间抗压强度相比，差异无显著性意义（P〉0．05）。结论：水牛角胎经H2O2浸渍48h处理，保留了较好的生物力学强度。

Effect on the mechanical characteristics of matrix in buffalo cornu soaked by hydrogen peroxide

BACKGROUND: Previous research reveals that, the morphous, structure, physicochemical property and mechanical characteristics of matrix in buffalo cornu all contribute to the biomaterials in the field of orthopaedics.OBJECTIVE: To investigate the correlation between the duration of matrix in buffalo cornu soaked in 30% hydrogen peroxide and the antigenicity, the biomechanical intensity of matrix in buffalo cornu treated by deproteinization.DESIGN, TIME AND SETTING: Measurement experiments were performed in the Mechanics Laboratory, Kunming University of Science and Technology (Kunming, Yunnan, China) from February to March in 2000.MATERIALS: Buffalo was provided by Luxi Abattoir of Yunnan Province (China), and then processed into test pieces at a nominal thickness.METHODS: The metric test pieces were rinsed with distilled water and soaked by chloroform-methyl alcohol at a ratio of 1:1 for 48 hours, then in 30% hydrogen peroxide for 24 and 48 hours, which served as the experimental group. While those remaining untreated were taken as controls.MAIN OUTCOME MEASURES: The modulus of elasticity and compressive strength were measured by electric measurement method and direct pressurization method, respectively.RESULTS: The modulus of elasticity between the experimental group and the control group had no significant difference (P>0.05). The compressive strength in the control group was significantly higher than that in the experimental group (P<0.01), and there were no significant differences between the experimental group treated for 24 hours and the experimental group treated for 48 hours (P>0.05).CONCLUSION: Subsequent to soaking in 30% hydrogen peroxide for 48 hours, the matrix in buffalo cornu maintains better biomechanicai intensity.