| HSV-Ⅰ亚单位疫苗新制备方法的初步探讨 |
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| 引用本文： | 胡丽萍,陈晓,梁浩麟,陈保平,陈敏诲,姚学军.HSV-Ⅰ亚单位疫苗新制备方法的初步探讨[J].武汉大学学报(医学版),1992(2). |
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| 作者姓名： | 胡丽萍 陈晓 梁浩麟 陈保平 陈敏诲 姚学军 |
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| 作者单位： | 湖北医学院基础实验中心电镜室，湖北医学院病毒所细胞生物室，湖北医学院基础实验中心电镜室，湖北医学院基础实验中心电镜室，湖北医学院病毒所细胞生物室，湖北医学院病毒所细胞生物室 |
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| 摘 要： | 本实验从Ⅰ型单纯疱疹病毒(HSV-1)感染的 Hep-2细胞提取单纯疱疹病毒的亚单位疫苗。以去污剂 NP-40溶解下病毒的亚单位以及与细胞膜结合的病毒特异性糖蛋白,用超速离心的方法将其分离出来。经免疫双扩散方法检测.提取物亚单位疫苗显示了特异的抗原性。疫苗接种小鼠之后,以间接免疫荧光技术测得小鼠血清中出现的 HSV-1抗体。
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| 关 键 词： | 疱疹病毒感染 疫苗 减毒/免疫 疫苗 减毒/分离与提纯 |
The Primary Study of New Preparation Method of HSV-1 Subunit Vaccine |
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| Hu Liping Liang Haolin Laboratory of Electron Microscope of Basic Medical Experiment Center,Chen Xiao Virus Research Institute,Hubei Medical College,Wuhan ,China.The Primary Study of New Preparation Method of HSV-1 Subunit Vaccine[J].Medical Journal of Wuhan University,1992(2). |
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| Authors: | Hu Liping Liang Haolin Laboratory of Electron Microscope of Basic Medical Experiment Center Chen Xiao Virus Research Institute Hubei Medical College Wuhan China |
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| Institution: | Hu Liping Liang Haolin Laboratory of Electron Microscope of Basic Medical Experiment Center,Chen Xiao Virus Research Institute,Hubei Medical College,Wuhan 430071,China |
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| Abstract: | Antiherpes subunit vaccine was prepared from Hep-2 cells infected with herpes simplex virus type-1(HSV-1).Virus subunit and membranebound virus-specific glycoproteins were sotubilized with Nonidet P-40 and separated by ultracentifugation.The antigenic property of the vaccine was detected with immuodiffusion tests.The antibody against HSV-1 in mice immunized with subunit vaccine was tested with inderect immunoflorescence technoque show the antibody againsted HSV-1 in mice sera was developed. |
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| Keywords: | MeSH herpesvirus infections vaccines attenuated/IM vaccines attenuated/IP |
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