Coxsackievirus B3m与T-2毒素对小鼠脂质过氧化物代谢的影响

目的 探讨Ｔ－２毒素与ＣｏｘｓａｃｋｉｅｖｉｒｕｓＢ３ｍ的双重作用小鼠肝脏脂质过氧化物的影响。方法 实验采用析因设计,ＢＡＬＢ／Ｃ小鼠随机分为４组,Ｉ正常对照组,ＩＩ病毒组,ⅢＴ－毒素组,Ⅳ病毒＋毒素组,病毒组常腹腔接种０．１ｍｌ内含１０００ＴＣＩＤ５０的１６４０营养液,毒素组隔日按１．０ｍｇ／ｋｇ体重Ｔ－２毒素灌胃,直至实验结束,病毒＋毒素组先Ｔ－２灌胃,３周后腹腔接种同稀释度病毒,各组鼠于病毒

Combined effect of Coxsackievirus B3m and T-2 toxin on metabo lism of lipid peroxide in mice

Objective To research the combined effect of Coxsackievirus B 3m and T 2 toxin on the metabolism of lipid peroxide by factorial design.Methods BALB/C mice as experimental animals were randomly divided into 4 groups:1.control group;2.virus group;3.T 2 toxin group;4.virus and T 2 toxin group.The mice in group 2 were injected with Coxsackievirus B 3m liquor 1000 TCID 50 in the dose of 0.1ml into abdominal cavity.The mice in group 3 were forced to swallow T 2 toxin every other day in the dose of 1.0mg/kg until the end of the experiment.The mice in group 4 were treated with T 2 toxin in the same way in the first 3 weeks,then the same concentration viral liquor like that used in group 2 was inoculated.The content of LPO in 4 groups was determined when the mice were killed in the seventh,fourteenth,twentieth,thirtieth and fiftieth day after inoculation.Results The results show that both virus and T 2 toxin have significant influence on the content of LPO in mice liver.The interaction between virus and T 2 toxin was not remarkable.Conclusions The obvious lipid peroxidation induced by coxsackievirus B 3m and T 2 toxin is more serious than that caused by Coxsackievirus B 3m or T 2 toxin respectively.