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| 流感病毒A/FM/47(H1N1)血凝素基因克隆、序列分析 | |
| 引用本文： | 王艳芳,闵晓莉,杨子峰,何金洋,孙立,王新华.流感病毒A/FM/47(H1N1)血凝素基因克隆、序列分析[J].热带医学杂志,2007,7(12):1153-1154,1193. |
| 作者姓名： | 王艳芳 闵晓莉 杨子峰 何金洋 孙立 王新华 |
| 作者单位： | 1. 华南师范大学激光运动医学实验室,广州,510006 2. 广东省中医院,广州,510120 3. 广州医学院第一附属医院,广州呼吸疾病研究所,广州,510120 4. 广州中医药大学,广州,510405 5. 暨南大学,广州,510630 |
| 摘 要： | 目的对流感病毒A/Font Monmouth/47(H1N1)血凝素基因进行变异分析。方法用SPF鸡胚增殖毒株,提取病毒基因组总RNA,应用RT-PCR技术扩增该病毒株的HA基因,并克隆到pMD18-T载体上,测定HA基因核苷酸序列。结果该毒株的HA基因全长为1677bp,含有完整的阅读框架,编码545个氨基酸;BLAST序列比对结果显示,该毒株HA基因与其他H1基因核苷酸的同源性为98%。结论该毒株HA核苷酸序列发生了变异,出现多处碱基替换,可能是病毒毒力增强的原因。 |
| 关 键 词： | 流感病毒 血凝素 基因序列 |
| 文章编号： | 1672-3619（2007）12-1153-02 |
| 收稿时间： | 2007-07-13 |
| 修稿时间： | 2007-09-06 |
Cloning and Sequencing of HA Gene of Avian Influenza Virus Strain A/Font Monmouth/47 ( H1N1 ) |
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| WANG Yan-fang,MIN Xiao-li,YANG Zi-feng,HE Jin-ying,SUN Li,WANG Xin-hua.Cloning and Sequencing of HA Gene of Avian Influenza Virus Strain A/Font Monmouth/47 ( H1N1 )[J].Journal Of Tropical Medicine,2007,7(12):1153-1154,1193. | |
| Authors: | WANG Yan-fang MIN Xiao-li YANG Zi-feng HE Jin-ying SUN Li WANG Xin-hua |
| Abstract: | Objective To clone the hemagglutinin(HA) gene from influenza A(H1N1).Method HA gene was amplified using specific primers and RT-PCR according to the sequence published on Genbank.The amplified fragment was cloned into pMD18-T vector for sequence analysis.Result The cloned HA gene shared 98% nucleotide sequence homology with the other H1N1 subtypes.Conclusion The results confirmed the high frequency of recombination of the influnenza A virus genome and the high level of genetic variation variation of Influenza A. |
| Keywords: | influenza A HA gene sequence |
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