| Infrequent microsatellite instability mutator phenotype in Chinese hepatocellular carcinomas |
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| 作者姓名： | 方丽 房殿春 汪荣泉 杨仕明 吴凯 |
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| 作者单位： | DepartmentofGastroenterology,SouthwestHospital,ThirdMilitaryMedicalUniversity,Chongqing,400038,China |
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| 摘 要： | Objective: In order to elucidate the molecular mechanisms that might be responsible for hepatocarcinogenesis, we examined microsatellite instability (MSI), mismatch repair gene hMLH1 mutation and methylation in hepatocellular carcinoma. Methods: Fifty-two cases of surgically resected sporadic hepatocellular carcinoma (HCC) were studied, hMLH1 mutation was examined by two-dimensional electrophoresis and DNA sequencing; hMLH1 methylation was determined by methylation-specific PCR (MSP); and MSI at BAT26 was analyzed by PCR-based methods. Results: MSI at BAT26 was found in 3 of 52 cases (5.8%) of the tumors analyzed. No hMLH1 mutation or hypermethylation was found in these 52 cancerous tissues. No correlation existed between MSI and clinico-pathological characteristics of the patients. Conclusion: Our results suggest that MSI at BAT26 is rarely associated with carcinogenesis of chinese HCC. The genesis of sporadic HCC may occur in an alternative pathway that is probably different from MSI pathway.
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| 关 键 词： | 肝细胞癌 微卫星不稳定性 基因突变 基因型 聚合酶链反应 |
Infrequent microsatellite instability mutator phenotype in Chinese hepatocellular carcinomas |
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| FANG Li,FANG Dian-chun WANG Rong-quan YANG Shi-ming WU Kai.Infrequent microsatellite instability mutator phenotype in Chinese hepatocellular carcinomas[J].Journal of Medical Colleges of PLA(China),2003,18(6):341-344,354. |
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| Authors: | FANG Li FANG Dian-chun WANG Rong-quan YANG Shi-ming WU Kai |
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| Institution: | FANG Li,FANG Dian-chun WANG Rong-quan YANG Shi-ming WU Kai Department of Gastroenterology,Southwest Hospital,Third Military Medical University,Chongqing,400038,China |
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| Abstract: | Objective: In order to elucidate the molecular mechanisms that might be responsible for hepatocarcinogenesis, we examined microsatellite instability (MSI), mismatch repair gene hMLH1 mutation and methylation in hepatocellular carcinoma. Methods: Fifty-two cases of surgically resected sporadic hepatocellular carcinoma (HCC) were studied. hMLH1 mutation was examined by two-dimensional electrophoresis and DNA sequencing; hMLH1 methylation was determined by methylation-specific PCR(MSP); and MSI at BAT26 was analyzed by PCR-based methods. Results: MSI at BAT26 was found in 3 of 52 cases (5.8%) of the tumors analyzed. No hMLH1 mutation or hypermethylation was found in these 52 cancerous tissues. No correlation existed between MSI and clinico-pathological characteristics of the patients. Conclusion: Our results suggest that MSI at BAT26 is rarely associated with carcinogenesis of chinese HCC. The genesis of sporadic HCC may occur in an alternative pathway that is probably different from MSI pathway. |
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| Keywords: | hepatocellular carcinoma hMLH1 mutation and methylation microsatellite instability |
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