大豆苷元对人非小细胞肺癌A549、H1299细胞增殖、迁移能力的影响及机制

目的探究大豆苷元(daidzein,Daid)对非小细胞性肺癌细胞株A549和H1299增殖、迁移能力的影响及可能机制。方法以A549和H1299为研究对象,CCK-8法检测Daid(0,5,10,25,50,100,200μmol·L^-1)抑制A549和H1299两种细胞的增殖情况。划痕和Transwell小室实验检测Daid对肺癌细胞迁移和侵袭能力的影响。Western blot检测Cleaved Caspase-3和LC3Ⅱ/Ⅰ等的蛋白表达。结果与对照组相比,Daid在体外可明显抑制肺癌细胞的增殖,且抑制作用呈浓度依赖关系。Daid(100μmol·L^-1)可明显降低人肺癌细胞A549和H1299的迁移侵袭能力。Western blot结果显示,Daid(100μmol·L^-1)预处理后,两种肺癌细胞的Cleaved Caspase-3及LC3Ⅱ/Ⅰ表达升高。结论Daid可抑制人非小细胞肺癌A549和H1299细胞的增殖,使上述细胞的迁移侵袭能力明显下降,其机制可能与Daid诱导细胞凋亡及自噬有关。

Effect of daidzein on proliferation and migration of lung cancer cells and its related mechanism

Aim To investigate the effect of Daidzein(Daid)on proliferation and migration of non-small cell lung cancer cell lines A549 and H1299 and its possible mechanism.Methods CCK-8 was adopted to detect cell proliferation of A549 and H1299 cells inhibited by Daid(0,5,10,25,50,100,200μmol·L^-1).The effect of Daid on proliferation and migration of lung cancer cells was detected by scratch healing and Transwell chamber assay.The protein expressions of cleaved Caspase-3 and LC3Ⅱ/Ⅰwere detected by Western blot.Results Daid had significantly inhibitory effects on the proliferation of lung cancer cells in vitro in a concentration-dependent manner when compared with control group.Daid(100μmol·L^-1)could inhibit the migration and invasion ability of human lung cancer cells A549 and H1299.Western blot results showed that the expressions of cleaved Caspase-3 and LC3Ⅱ/Ⅰwere elevated after Daid pretreatment.Conclusions Daid can inhibit the proliferation of human non-small cell lung cancer cells A549 and H1299,and reduce the migration and invasion of cells.The mechanism may be related to cell apoptosis and autophagy induced by Daid.