实时荧光定量PCR法检测淋病奈瑟菌感染的效果

①目的 应用实时荧光定量聚合酶链反应(FQ-PCR)技术准确定量检测临床标本中淋病奈瑟菌基因，同时与金标准—细菌培养法比较其灵敏度和特异度，为临床淋病的诊疗提供依据。②方法 采集l73例病人临床标本，以PE5700全自动PCR扩增分析仪和淋病奈瑟菌实时荧光定量PCR检测试剂盒进行核酸检测。同时，用含PolyViteX的巧克力板对淋病奈瑟菌进行分离，用ATB Expression自动细菌鉴定仪及配套的APINH进行鉴定。③结果 173份临床标本中，荧光定量PCR法示47例淋病奈瑟菌阳性，阳性率为27．2％；细菌培养法示41例淋病奈瑟菌阳性，阳性率为23．7％。与细菌培养法相比，荧光定量PCR法的灵敏度为100％，特异度为95．5％，两者的符合率为96．5％。④结论 荧光定量PCR技术具有简便、快捷、灵敏度高、特异度高、定量准确等优点，在临床检测淋病奈瑟菌中具有良好的应用前景。

REAL-TIME FLUORESCENCE QUANTITATIVE POLYMERASE CHAIN REACTION FOR DETECTION OF NEISSERIA GONORRHOEAE INFECTION

Objective To detect Neisseria gonorrhoeae DNA in genitourinary specimens by real-time fluorescence quantitative Polymerase Chain Reaction (FQ-PCR), and to provide a clinical basis for the diagnosis and treatment of gonorrhoea. Methods The study consisted 173 specimens, which were detected for DNA with Neisseria gonorrhoeae real-time FQ-PCR. The specimens were cultured as well. Results Of the 173 clinical specimens, real-time FQ-PCR showed that 47 were positive(27.2%);while 41 cases(23.7%) were positive by the culture method. Compared to the culture method, the specificity, sensitivity, and agreement of FQ-PCR was 95.5%, 100%, and 96.5%, respectively. Conclusion FQ-PCR is a simple, rapid, sensitive and specific method to detect Neisseria gonorrhoeae.