Characterization, anti-oxidative and anti-inflammatory effects of Costa Rican noni juice (Morinda citrifolia L.) |
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Authors: | E DussossoyP Brat E Bony F BoudardP Poucheret C MertzJ Giaimis A Michel |
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Institution: | a Laboratoire de Pharmacologie et Physiopathologie Expérimentales, UMR Qualisud, Faculté de Pharmacie, Université Montpellier I, 15 avenue Charles Flahault, 34000 Montpellier, France b Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Département PERSYST, UMR Qualisud, TA B-95/16, 34398 Montpellier Cedex 5, France c Laboratoire d’immunologie, Faculté de Pharmacie, Université Montpellier I, 15 Avenue Charles Flahault, 34000 Montpellier, France |
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Abstract: | Aim of the studyNoni fruit (Morinda citrifolia L.) juice has been used for more than 2000 years in Polynesia as a traditional folk medicine. The aim of the present study was to finely characterize noni juice from Costa Rica and to evaluate its anti-oxidative and anti-inflammatory activities.Materials and methodsA microfiltrated noni juice was prepared with Costarican nonis. HPLC-DAD and Electro Spray Ionization Mass Spectrometric detection (HPLC-ESI-MS) were used to identify phenolic compounds and iridoids. The anti-oxidative activity of noni juice was measured in vitro by both Oxygen Radical Absorbance Capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging methods. The anti-inflammatory effects of noni juice were investigated in vitro by: measuring its effect on nitric oxide and prostaglandin E2 production by activated macrophages, evaluating its inhibitory activities on cyclooxygenase (COX)-1 and -2 and in vivo on a carrageenan-induced paw oedema model in rats.ResultsSeveral polyphenols belonging to the coumarin, flavonoid and phenolic acid groups, and two iridoids were identified. Noni juice demonstrated a mean range free radical scavenging capacity. Furthermore, it also reduced carrageenan-induced paw oedema, directly inhibited cyclooxygenase COX-1 and COX-2 activities and inhibited the production of nitric oxide (NO) and prostaglandins E2 (PGE2) in activated J774 cells, in a dose dependent manner.ConclusionsThis study showed that noni's biological effects include: (1) anti-oxidant properties probably associated with phenolic compounds, iridoids and ascorbic acid and (2) anti-inflammatory action through NO and PGE2 pathways that might also be strengthened by anti-oxidant effects. |
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Keywords: | Noni Morinda citrifolia L Rubiaceae LC-MS Phenolic compounds Anti-oxidative activity Anti-inflammatory activity |
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