| Developmental changes of glutamate acid decarboxylase 67 in mouse brain after hypoxia ischemia |
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| 引用本文： | Fa-Lin XU,Chang-Lian ZHU,Xiao-Yang WANG Department of Pediatrics,the Third Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China. Developmental changes of glutamate acid decarboxylase 67 in mouse brain after hypoxia ischemia[J]. 中国神经科学杂志, 2006, 0(1) |
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| 作者姓名： | Fa-Lin XU Chang-Lian ZHU Xiao-Yang WANG Department of Pediatrics the Third Affiliated Hospital of Zhengzhou University Zhengzhou 450052 China |
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| 作者单位： | Fa-Lin XU，Chang-Lian ZHU，Xiao-Yang WANG Department of Pediatrics，the Third Affiliated Hospital of Zhengzhou University，Zhengzhou 450052，China |
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| 摘 要： | Objective To study the developmental changes of glutamic acid decarboxylase-67 (GAD-67, a GABA synthetic enzyme) in normal and hypoxic ischemic (HI) brain. Methods C57/BL6 mice on postnatal day (P) 5, 9, 21 and 60, corresponding developmentally to premature, term, juvenile and adult human brain were investigated by using both Western blot and immunohistochemistry methods either in normal condition or after hypoxic ischemic insult. Results The immunoreactivity of GAD67 was up regulated with brain development and significant difference was seen between mature (P21, P60) and immature (P5, P9) brain. GAD67 immunoreactivity decreased in the ipsilateral hemisphere in all the ages after hypoxia ischemia (HI) insult, but, significant decrease was only seen in the immature brain. Double labeling of GAD67 and cell death marker, TUNEL, in the cortex at 8h post-HI in the P9 mice showed that (15.6±7.0)% TUNEL positive cells were GAD67 positive which was higher than that of P60 mice. Conclusion These data suggest that GABAergic neurons in immature brain were more vulnerable to HI insult than that of mature brain.
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Developmental changes of glutamate acid decarboxylase 67 in mouse brain after hypoxia ischemia |
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| Fa-Lin XU,Chang-Lian ZHU,Xiao-Yang WANG. Developmental changes of glutamate acid decarboxylase 67 in mouse brain after hypoxia ischemia[J]. Neuroscience Bulletin, 2006, 0(1) |
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| Authors: | Fa-Lin XU Chang-Lian ZHU Xiao-Yang WANG |
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| Affiliation: | Fa-Lin XU,Chang-Lian ZHU,Xiao-Yang WANG Department of Pediatrics,the Third Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China |
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| Abstract: | |
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| Keywords: | glutamic acid decarboxylase brain development hypoxia-ischemia |
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