| 地高辛标记探针原位杂交法检测肝病患者外周血单个核细胞中TTV-DNA |
| |
| 引用本文： | 刘暘,其其格. 地高辛标记探针原位杂交法检测肝病患者外周血单个核细胞中TTV-DNA[J]. 标记免疫分析与临床, 2002, 9(3): 135-137. DOI: 10.3969/j.issn.1006-1703.2002.03.004 |
| |
| 作者姓名： | 刘暘 其其格 |
| |
| 作者单位： | 内蒙古医学院第一附属医院传染病教研室,内蒙古,呼和浩特,010050;内蒙古医学院第一附属医院传染病教研室,内蒙古,呼和浩特,010050 |
| |
| 摘 要： | 为检测肝病患者外周血单个核细胞(PBMC)中TTV-DNA(输血传播病毒脱氧核糖核酸), 以TTV ORF1为模板, 应用地高辛(Dig)作为标记物, 经聚合酶链反应(PCR)制备探针, 建立原位杂交方法进行检测.结果显示: 血清TTV-DNA阳性组, 双链探针检测PBMC中TTV-DNA, 阳性检出率为58.06%(18/31); 血清TTV-DNA阴性组, 双链探针检测PBMC中TTV-DNA, 阳性检出率为27.59%(8/29).双链探针阳性者, 再以负链探针检测其复制情况, 阳性率为22.2%(4/18). 结论: TTV可感染PBMC并在PBMC中复制.
|
| 关 键 词： | TT病毒 原位杂交 聚合酶链反应 |
| 文章编号： | 1006-1703(2002)03-0135-03 |
| 修稿时间： | 2001-10-30 |
Detection of TTV-DNA in PBMC Using Digoxigenin Labelled Probe by in Situ Hybridization |
| |
| LIU Yang,QiQi-ge. Detection of TTV-DNA in PBMC Using Digoxigenin Labelled Probe by in Situ Hybridization[J]. Labeled Immunoassays and Clinical Medicine, 2002, 9(3): 135-137. DOI: 10.3969/j.issn.1006-1703.2002.03.004 |
| |
| Authors: | LIU Yang QiQi-ge |
| |
| Abstract: | To determine TTV-DNA in PBMC in patients with viral hepatitis, a study of in situ hybridization using digoxigenin labelled probe by PCR method to the TTV ORF1 region was performed on PBMC. Resullts showed that the detection rate of TTV-DNA using double-stranded probe in TTV-DNA positive group in sera was 58.06(18/31), and the detection rate of TTV-DNA using double-stranded probe in TTV-DNA negative group in sera was 27.59(8/29).For TTV-DNA positive group detected by double-stranded probe, then we use negative-stranded probe to detect their replication. The detection rate was 22.2%(4/18). Conclusions: TTV can infect PBMC and replicate in PBMC. |
| |
| Keywords: | TT virus In situ hybridization Polymerase chain reaction |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
