槌果藤提取物治疗硬皮病动物模型的研究

目的 观察槌果藤乙酸乙酯提取物、乙醇提取物对硬皮病小鼠模型真皮厚度、Ⅰ型、Ⅲ型胶原合成及转化生长因子-β1表达的影响。方法 用博莱霉素致小鼠硬皮病模型，给予槌果藤乙酸乙酯提取物、乙醇提取物外用治疗；2、4、6周末皮肤病理观察真皮厚度变化，免疫组化观察Ⅰ型、Ⅲ型胶原及转化生长因子-β1的合成表达。结果 硬皮病小鼠模型外用槌果藤乙酸乙酯提取物、乙醇提取物2、4、6周后，与未用药的模型组比较，真皮厚度明显减少（F = 12.99，P < 0.01），Ⅰ型胶原（F = 7.47，P < 0.01）及转化生长因子-β1表达降低（F = 11.76，P < 0.01），对Ⅲ型胶原影响不大。结论 槌果藤乙酸乙酯提取物、乙醇提取物具有抗皮肤纤维化作用。

Efficacy of Capparis Spinosa extracts in the treatment of scleroderma:an experimental study in animal models

Objective To observe the effect of ethanol extract and ethyl acetate extract of Capparis Spinosa on the thickness of dermis,synthesis of collagen type Ⅰ,type Ⅲ,and expression of transforming growth factor-β1 in mouse models of scleroderma.Methods Mouse models of scleroderma were established through local injection of bleomycin on the back once a day for 4 weeks.After confirmation of model establishment,72 mouse models were equally and randomly divided into three groups.Two groups received topical treatment with ethanol extract of Capparis Spinosa and ethyl acetate extract of Capparis Spinosa,respectively,no treatment was given to the rest of the control group.After 2-,4-,6-week treatment,8 mice were sacrificed and tissue samples were obtained from the back,and subiected to the measurement of dermal thickness by HE staining,as well as to the analysis of expression of collagen type Ⅰ,collagen type Ⅲ and transforming growth factor-β1 by immunohistochemical staining.Results On week 2,4,6,the thickness of dermis was 23.22,24.94,19.97 μm respectively in mice treated with ethanol extract of Capparis Spinosa,27.66.26.15,22.13 μm respectively in those treated with ethyl acetate extract of Capparis Spinosa.Compared with the mouse models without treatment,the thickness of dermis significantly decreased(F=12.99,P＜0.01),the expression of collagen type Ⅰ(F=7.47,P＜0.01)and transforming growth factor-β1(F=11.76,P＜0.01)were also inhibited in those receiving treatment.However,the expression of collagen type Ⅲ was not affected obviously by the treatment.Conclusion The ethanol extract and ethyl acetate extract of Capparis Spinosa have the effect against skin fibrosis.