工人尿中三硝基甲苯及五种代谢物的质谱鉴定和高效液相色谱测定

为阐明TNT对肝脏损伤的机理,搞清尿中代谢物的种类,结构及含量有着十分重要的意义。将工人尿样加酸水解使代谢物游离,用苯提取液做色谱/质谱分析。鉴定出两个-氨基二硝基甲苯(4-A,2-A);两个二氨基-硝基甲苯(2,4-DA,2,6-DA)。又用化学电离源质谱/质谱法鉴定出易分解的羟氨基二硝基甲苯(4-HA)。用高效液相色谱法测定了某化工厂9名工人尿样。其含量顺序为4-A,4-HA,2-A,2,4-DA,2,6-DA。大多数尿样未检出TNT原形。4-A浓度最大值7.7mg/L,仍低于ILO规定的生物阈限值。

MASS SPECTROGRAPHIC IDENTIFICATION AND HPLC DETERMINATION OF TNT AND FIVE METABOLITES IN URINE OF WORKERS

In order to explian the mechanism of liver damage by TNT, it is of important significance to identify the structure of metabolites and to determine their concentrations in urine. Urine samples of workers were hydrolysed in acidic condition to set free the metabolites. Benzene extracts were analyzed by GC/MS. Two amino-dinitrotoluene(4-A,2-A)and two diamino - nitro-toluene (2,4-DA, 2,6-DA) were identified. Hydroxyamino - dinitrotoluene (4-HA) would decomposed in inlector of GC, it was identified by MS/CI. Nine urine samples of exposed workers were analyzed by HPLC. The sequence of concentrations were: 4A, 4-HA, 2-A, 2, 4-DA and 2, 6-DA. Original form TNT was not detected in most samples. Maximum concentration of 4-A was 7.7 mg/L. Still lower than BEL of ILO.