| 氧化砷对NB4和Jurkat细胞周期的影响 |
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| 引用本文： | 王韫芳,孙红琰,王全立,李昕权.氧化砷对NB4和Jurkat细胞周期的影响[J].白血病．淋巴瘤,2002,11(3):132-134. |
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| 作者姓名： | 王韫芳 孙红琰 王全立 李昕权 |
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| 作者单位： | 1. 军事医学科学院附属医院，北京 100039;2. 军事医学科学院野战输血研究所，北京 100850 |
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| 摘 要： | 目的：研究氧化砷(As2O3)对白血病细胞系NB4、Jurkat细胞周期的影响及对细胞周期及凋亡相关蛋白的调节作用。方法：以MTT、基因组DNA电泳、蛋白／DNA双参数流式细胞术等方法研究As2O3对白血病细胞的作用及机制。结果：随着As2O3作用时间的延长，白血病细胞增殖明显受到抑制，DNA电泳出现“梯”状条带，流式细胞术检出亚G1峰，细胞周期阻滞于G1和G2／M期，部分细胞周期及凋亡相关的调控蛋白表达改变。结论：As2O3通过改变细胞周期及凋亡相关蛋白的表达而抑制白血病细胞增殖和诱导其凋亡。
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| 关 键 词： | 砷剂 白血痛细胞系 细胞周期 增殖 凋亡 |
| 文章编号： | 1009-9921(2002)03-0132-03 |
| 修稿时间： | 2001年5月15日 |
Preliminary Studies on The Effect of Arsenic Trioxide on Proliferation and Apoptosis of Leukemic Ceils and It's Mechanism |
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| WANG Yun fang,SUN Hong yan,WANG Quan li,et al..Preliminary Studies on The Effect of Arsenic Trioxide on Proliferation and Apoptosis of Leukemic Ceils and It''''s Mechanism[J].Journal of Leukemia & Lymphoma,2002,11(3):132-134. |
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| Authors: | WANG Yun fang SUN Hong yan WANG Quan li |
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| Institution: | WANG Yun fang,SUN Hong yan,WANG Quan li,et al.Research Clinic,Academy of Military Medical Sciences |
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| Abstract: | Objective:To investigate the effect of arsenic trioxide (As 2O 3) on proliferation,cell cycle regulation and apoptosis in leukemic cell lines and it's mechanism.Methods:MTT assay,electrophoresis of genomic DNA,as well as protein/DNA multiparameter flow cytometry were used.Results:Cell proliferation were significantly inhibited in a dose and time dependent manner.Apoptosis were induced in these cells.Furthermore,As 2O 3 could change the content of some cell cycle and apoptosis related proteins in NB 4/Jurkat cells in which both G 1 and G 2/M phases were arrested.Conclusion:The change of all cycle regulatory protein may play an improtant role in proliferation inhibition and apoptosis of leukemic cells regulatery from As 2O 3. |
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| Keywords: | Arsenicals Leukemic cell lines Cell cycle Proliferation Apoptosis |
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