硫熏和直接热风加热的粉葛中葛根素含量及药效的比较研究

为考察硫熏及直接热风加热的粉葛中葛根素含量及药效的差异,将鲜粉葛削皮、切小块分别制备成晒干、直接热风干燥及硫熏后热风干燥样品,分别测定样品水分含量。采用HPLC,比较晒干、直接热风干燥及硫熏后热风干燥样品中粉葛中主要药效成分葛根素的含量;建立醉酒模型,以SOD含量为指标,灌胃给药硫熏和非硫熏粉葛样品的水煎煮提取物,比较硫熏对粉葛提高醉酒模型小鼠肝脏、血清中SOD含量的影响;同时考察不同包装及贮存条件下,硫熏及非硫熏样品的霉变及虫蛀情况。结果显示硫熏后的粉葛样品,其葛根素含量发生明显变化,随着硫熏次数及硫磺用量的提高,其葛根素含量逐渐下降。且硫熏可显著降低粉葛提高的醉酒模型小鼠肝脏、血清中SOD含量,与晒干及直接热风干燥组均呈显著性差异。水分符合药典标准的硫熏样品和直接热风干燥样品均未发生虫蛀和霉变。研究显示硫熏可显著降低粉葛中主要药效成分的含量,降低粉葛的药效,表明硫熏后粉葛质量显著下降。而未硫熏直接热风干燥的样品其主要药效成分的含量、药效以及贮藏结果均与晒干相似,是一种较好的产地干燥工艺,可推广。

Comparison of sulfur fumigation processing and direct hot air heating technology on puerarin contents and efficacy of Puerariae Thomsonii Radix

In order to compare the effect of sulfur fumigation processing and direct hot air heating technology on puerarin contents and efficacy of Puerariae Thomsonii Radix, the fresh roots of Pueraria thomsonii were cut into small pieces and prepared into direct sunshine drying samples, direct hot air drying samples, and sulfur fumigation-hot air drying samples. Moisture contents of the samples were then determined. The puerarin contents of different samples were compared by HPLC method. Moreover, the models of drunkenness mice were established, and then with superoxide dismutase (SOD) content as the index, aqueous decoction extracts of Puerariae Thomsonii Radix samples with sulfur fumigation processing and non-sulfur fumigation processing methods were administrated by ig; the effects of sulfur fumigation on contents of SOD in mice liver and serum were determined, and the sulfur fumigation samples and non-sulfur fumigation samples were investigated for moth and mildew under different packaging and storage conditions. Results showed that the sulfur fumigation samples significantly changed the puerarin content from Puerariae Thomsonii Radix. The content of puerarin was decreased gradually when increasing the times of sulfur fumigation and amount of sulfur. SOD content in drunken mice liver and serum was significantly decreased when increasing the times of sulfur fumigation, showing significant difference with both direct sunshine drying group and direct hot air drying group. Moth and mildew were not found in the sulfur fumigation samples and direct hot air drying samples whose moisture contents were lower than the limit in Pharmacopoeia. Research showed that sulfur fumigation can significantly reduce the content of main active ingredients and reduce the efficacy of Puerariae Thomsonii Radix, indicating that the quality of Puerariae Thomsonii Radix was significantly decreased after sulfur fumigation. However, the contents of the main active ingredients, efficacy and storage results of the direct hot air drying samples were similar to those in direct sunshine drying samples, so the hot air drying process was a nice drying technology which could be promoted for use.