三种梅毒血清学检验方法在临床中的应用

目的：筛选有效的检测试剂,提高临床诊断符合率。方法：选取2011年12月-2012年12月的职业健康体检10286例标本,采用对酶联免疫吸附试验（ELISA）、甲苯胺红不加热血清试验（TRUST）、梅毒螺旋体明胶颗粒凝集试验（TPPA）3种血清学检测方法进行梅毒诊断,并作结果分析。结果：ELISA法检出阳性标本675例中,经TPPA确证阳性657例,阳性符合率97.33%。TRUST法检出阳性424例,均经TPPA确证阳性;而ELISA筛查阳性而TRUST阴性的标本有7.17%的假阳性结果。结论：建议3种检测方法结合应用能更有效提高梅毒临床诊断符合率。

Clinical Application of Three Kinds of Serological Testing Methods for Syphilis

Objective：To screen effective detection reagent and increase clinical diagnostic coincidence rate.Method：10 286 cases of specimens participated in health examination from December 2011 to December 2012 were selected for diagnosis of syphilis and result analysis using 3 serological tests including enzyme-linked immunosorbent assay（ELISA）,toluidine red unheated serum test（TRUST）and treponema pallidum gelatin particle agglutination test（TPPA）.Result：In the 675 cases detected positive by ELISA,657 cases were confirmed positive by TPPA and the positive coincidence rate was 97.33%.Four hundred and twenty-four cases were detected positive by TRUST,which were all confirmed positive by TPPA;however,there were 7.17% false positive results that were screened positive by ELISA but negative by TRUST.Conclusion：It is suggested to combine the 3 detection methods to effectively improve the clinical diagnostic coincidence rate of syphilis.