iNOS 、VEGF和CD34在人涎腺腺样囊性癌中的表达及意义

目的：研究诱导型一氧化氮合酶（inducible nitric oxidesynthase，iNOS）、血管内皮生长因子（vascular endo-thelial growth factOr，VEGF）及微血管密度（Microvessel density，MVD）在人涎腺腺样囊性癌（adenoid cystic carcinoma，ACC）中的表达及相关性，探讨它们与ACC血管生成和临床生物学行为的关系。方法：应用免疫组化SABC法，检测15例ACC、10例腮腺多形性腺瘤（plemorphic adenoma，PA）及10例正常腮腺组织（normal salivary gland，SG）中iNOS和VEGF的表达和微血管密度MVD（CD34标记）计数，并对上述指标应用SPSSl3．0统计软件进行统计学分析。结果：PA、SG、ACC中的iNOS和VEGF的阳性表达率及MVD计数依次增加，各组之间有显著性差异（P〈0．05）；MVD在ACC中的计数均随着iNOS和VEGF表达的增强而升高；iNOS和VEGF两者在腺样囊性癌之间也具有相关性。结论：iNOS、VEGF和CD34的表达与ACC的血管生成及发生发展密切相关。

Expression and Clinical Significance of iNOS,VEGF and CD34 in Human Adenoid Cystic Carcinoma of Salivary Glands

Objective: To study the expression of inducible nitric oxide synthase(iNOS),vascular endothelial growth factor(VEGF) and CD34,and their relations with angiogenesis and clinical biologic behavior in human adenoid cystic carcinoma(ACC) of salivary glands.Methods: SABC immunochemistry staining was applied to detect the expression of iNOS and VEGF;Microvessel density(MVD) was counted by anti-CD34 antibody in 15 cases of ACC,10 cases of pleomorphic adenoma(PA) and 10 cases of normal salivary gland.The data were ...