ZNF488对电离辐射诱发的鼻咽癌细胞CNE1侵袭迁移能力的影响

[目的]研究ZNF488 siRNA对电离辐射诱发的鼻咽癌CNE1侵袭迁移能力的影响.[方法]采用qRT-PCR和Western blot检测ZNF488 siRNA转染效率.采用划痕实验和Transwell侵袭实验检测电离辐射对鼻咽癌CNE1细胞侵袭迁移能力的影响.采用划痕实验和Transwell侵袭实验检测沉默ZNF488后,电离辐射诱发的CNE1细胞侵袭迁移能力是否改变.[结果]在mRNA和蛋白水平,实验组(siZNF488组)表达量均明显低于对照组(siRNA-Ctrl 组),其中实验组ZNF488 mRNA水平为对照组的(0.54±0.12)倍(P=0.023).划痕实验证明电离辐射明显增强鼻咽癌CNE1细胞迁移能力;Traoswell侵袭实验检测到0Gy组侵袭细胞数为302.67± 18.77,4Gy组侵袭细胞数为371.67± 15.63,4Gy组侵袭细胞数为0Gy组(1.23±0.03)倍(P=0.006).沉默ZNF488后,电离辐射诱发的CNE1细胞侵袭迁移能力明显减弱,这一功能的实现与上皮间质转化(EMT)进程的逆转息息相关.[结论]ZNF488 siRNA通过逆转EMT进程抑制鼻咽癌细胞CNE1电离辐射诱发的侵袭迁移能力.

Effect of ZNF488 on Ionizing Irradiation-induced Migration and Invasiveness of Nasopharyngeal Cancer CNE1 Cells

[Purpose] To investigate the effect of ZNF488 on ionizing irradiation (IR)-induced migration and invasion in nasopharyngeal cancer CNE1 cells.[Methods] ZNF488 siRNA was transfected into CNE1 cells,the expression of ZNF488 mRNA and protein was detected by qRT-PCR and Western blot after transfection,respectively.The transfected and non-transfected CNE1 cells were exposed to 200cGy/min X-ray.The invasion and migration of CNE1 cells were analyzed by Transwell invasion assay and scratch wound healing.[Results] The mRNA expression level of ZNF488 in transfected CNE1 cells was (0.54±0.12) times as in control group (P =0.023).Transwell test showed that the number of invasion cells in 4Gy IR group(371.67±15.63) was (1.23±0.03) times higher than that of 0Gy group(302.67±18.77).The expression of ZEB1 decreased and expression of E-eadherin increased in CNE1 cells after transfeeted with siZNF488,while the expression of ZEB1 increased and E-cadherin decreased after 4Gy IR.[Conclusion] Silencing ZNF488 could be a new target for inhibiting metastasis caused by IR,which may be associated with regulation of IR-induced epithelial mesenchymal transition (EMT).