麝香对骨缺损模型大鼠HMGB1和BMP2表达的影响

目的探讨麝香对高迁移率族蛋白1(highmobilitygroupbox-1protein,HMGB1)和骨形态发生蛋白2(bone morphogenetic protein-2,BMP2)在颅骨骨缺损模型大鼠组织中表达的变化。方法 SD大鼠300只,建立颅骨骨缺损模型。随机分为对照组和给药组,每组150只,再将这两个组按照第7、14和28天分为3个小组,每组50只。给药组按42 mg/kg给予天然麝香灌胃,对照组灌服等体积的生理盐水,均每日1次。第7、14和28天采用蛋白印迹法检测HMGB1和BMP2的表达。结果给药组HMGB1、BMP2在第7天的表达均达到峰值(P0.05),第14天时降低,第28天时表达最低;与对照组比较,第7天HMGB1、BMP2表达均有统计学意义(P0.05);给药组第7和14天HMGB1的表达明显高于本组第28天(P0.05)。结论麝香促进大鼠HMGB1、BMP2的表达增加,可能是颅骨骨缺损处的愈合原因之一。

Effects of musk on the expression HMGB1 and BMP2 in rat bone defect model

Objective To discuss the expression of high mobility group box 1 protein (HMGB1) and bone morphogenetic protein 2 (BMP2) in rat models of calvarial bone defect. Methods Skull bone defect model was established in 300 Sprague-Dawley rats and the rats were randomly divided into control group and treatment group, with 150 rats in each group. Each group was divided into 3 groups of 7d, 14d and 28d, with 50 rats in each group. The rats in the treatment group were given 42 mg/kg of natural musk, and the control group was given the same amount of normal saline, once per day. The expression of HMGB1 and BMP2 was detected by Western blotting at 7d, 14d and 28d. Results The expression of HMGB1 and BMP2 in the treatment group reached the peak on the 7th day (P<0.05), decreased on the 14th day and was the lowest on the 28th day. Compared with the control group, the expression of HMGB1 and BMP2 on the 7th day was significantly different (P<0.05). In the treatment group, the expression of HMGB1 on the 7th and 14th day was significantly higher than that of the 28th day (P<0.05). Conclusion Musk increased the expression of HMGB1 and BMP2 in rats, which may be one of the causes of skull bone defect healing.