Detection of human papillomavirus-related oral verruca vulgaris among Venezuelans |
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Authors: | G Premoli-de-Percoco I Galindo J L Ramirez M Perrone H Rivera |
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Institution: | Institute de Investigaciones "Raul Vicentelli", Facultad de Odontologia, Universidad Central de Venezuela, Caracas, Venezuela;Centro de Biologia Celular, Universidad Central de Venezuela, Caracas, Venezuela. |
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Abstract: | A sensitive in situ hybridization test under low stringency conditions (LCS) with a set of digoxigenin-labeled human papillomavirus mixed probes (D-L HPV MP) revelaed a positive reaction in 8 of 10 cases of oral verruca vulgaris (OVV), Ages ranged from 5 to 37 years with a mean of 14.5 years. 50% of all cases were located intraorally on the hard palate, followed in frequency by the commissures. These preliminary findings provide evidence of the role of HPV in OVV from a sample of the Venezuelan population. We show that in situ hybridization conducted under LSC is useful in HPV detection (regardless of the type) and the digoxigenin-labeling system is a rapid, relatively easy and specific method. In addition, this technique permits the retrospective evaluation of routinely processed material, thus widening the investigative spectrum for HPV. |
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Keywords: | human papillomavirus (HPV) in situ hybridization non-isotopic viral detection methods verruca vulgaris |
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