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| 紫外分光光度法测定海滩牵牛中总香豆素类成分的浓度 | |
| 引用本文： | 陈一村,蔡聪艺,冀宾,高分飞,张艳美,白雪婷,黄展勤,石刚刚. 紫外分光光度法测定海滩牵牛中总香豆素类成分的浓度[J]. 汕头大学医学院学报, 2010, 23(1): 5-6 |
| 作者姓名： | 陈一村 蔡聪艺 冀宾 高分飞 张艳美 白雪婷 黄展勤 石刚刚 |
| 作者单位： | 汕头大学医学院药理学教研室,广东,汕头,515041 |
| 基金项目： | 广东省科技计划资助项目，广东省中医药局资助项目，国家自然科学基金资助项目，广东省自然科学基金团队资助项目 |
| 摘 要： | 目的：测定草药海滩牵牛（IS）药材中总香豆素浓度。方法：采用紫外分光光度法，检测波长320nm，以伞形花内酯为对照品。结果：IS在2-32μg/mL质量浓度范围内，对照品吸光度和浓度线性关系良好（r=0．999）。平均回收率99．31％，相对标准偏差=1．88。结论：该法简便、可靠，为有效控制Is药材质量奠定了一定的基础。 |
| 关 键 词： | 海滩牵牛 总香豆素 紫外分光光度法 浓度，测定 |
Determination of Total Content of Coumarin in Ipomoea Stolonifera by Ultraviolet Spectrophotometry |
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| CHEN Yi-cun,CAI Cong-yi,JI Bin,GAO Fen-fei,ZHANG Yan-mei,BAI Xue-ting,HUANG Zhan-qin,SHI Gang-gang. Determination of Total Content of Coumarin in Ipomoea Stolonifera by Ultraviolet Spectrophotometry[J]. Journal of Shantou University Medical College, 2010, 23(1): 5-6 | |
| Authors: | CHEN Yi-cun CAI Cong-yi JI Bin GAO Fen-fei ZHANG Yan-mei BAI Xue-ting HUANG Zhan-qin SHI Gang-gang |
| Affiliation: | CHEN Yi-cun,CAI Cong-yi,JI Bin,GAO Fen-fei,ZHANG Yan-mei,BAI Xue-ting,HUANG Zhan-qin,SHI Gang-gang(Department of Pharmacology,Shantou University Medical College,Shantou 515041,China) |
| Abstract: | Objective： To determine the total content of coumarin in ipomoea stolonifera. Method： The cournarin in ipomoea stolonifera was detected at 320 nm by ultraviolet speetrophotometry. Results： There was a good linear relationship within the range of 2-32 μg/mL（r=0.999）. The average recovery was 99.31% （ n=3）with relative standard of 1.88%. Conclusion： This method is rapid, reproducible, accurate and can be used for detecting the total content of coumarin in ipomoea stolonifera. |
| Keywords: | ipomoea stolonifera total coumarin ultraviolet spectrophotometry content determination |
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