阿司匹林、盐酸氟桂利嗪联合预处理对SD大鼠局部脑缺血再灌注损伤的保护作用

目的采用Sprague-Dawley（SD）大鼠局灶性脑缺血再灌注模型，观察神经功能缺损评分、脑梗死体积及脑组织病理形态改变，探讨阿司匹林联合盐酸氟桂利嗪预处理对SD大鼠局灶性脑缺血再灌注损伤是否有保护作用。方法36只雄性健康sD大鼠，随机分为2组：对照组（n=18）、阿司匹林＋盐酸氟桂利嗪组（治疗组，n=18），各组按脑缺血90min再灌注3h（n=6）、6h（n=6）、24h（n=6）分为3个亚组。比较两组在相同再灌注各时间点神经功能缺损评分、脑梗死体积和脑组织病理形态的改变。结果治疗组相同再灌注各时间点神经功能缺损评分优于对照组（P〈0．05）；脑梗死体积明显小于对照组（P〈0．05）；与对照组相比，治疗组变性、坏死的神经元减少，空泡化改变减轻，组织间水肿减轻。结论阿司匹林联合盐酸氟桂利嗪预处理能减轻SD大鼠局灶性脑缺血再灌注损伤，减轻神经功能缺损症状，缩小梗死体积，减轻神经细胞变性、坏死及组织水肿。

Protective effect of pretreatment by combination of aspirin and flunarizine hydrochlorid on focal cerebral ischemia reperfusion damage in SD rat

Objective Applying focal ischemia-reperfusion model of SD rat,to observe the state of neurologic impairment score, cerebral pathomorphology and infarction volume after focal ischemia-reperfusion damage in SD rat. Methods 36 healthy male SD rats ( weight from 180 g to 220 g) were randomly divided into 2 groups: control group ( n = 18 ) and aspirin + flunarizine hydrochlorid ( namely treatment group n = 18 ). Each group was divided into 3 subgroups according to reperfusion 3 h( n = 6 ) ,6 h( n = 6 ) ,24 h (n = 6 )after ischemia for 90 minutes. The changes of neurologic impairment score, cerebral infarction volume and pathomorphology were compared at the same reperfusion time. Results There are great difference of the neurologic impairment score between two groups(P ＜0.05) ,the infarction volume can be seen at treatment group. The infarction volume in the treatment group was lower than that in the control group, P ＜ 0.05. In treatment group, the injury of cerebral tissue became serious with time went by, but compared with control group, the number of neuron which became degeneration and necrosis decreased, vacuolization and intertissue edema became relieved. Conclusion Pretreatment of combination of aspirin and flunarizine hydrochlorid can decrease focal ischemia-reperfusion damage, relieve the neurologic impairment symptoms;reduce infarction volume, reduce the neuron degeneration,necrosis and tissue edma.