血红素氧合酶-1对预防动脉粥样硬化及炎症的影响

目的:探讨血红素氧合酶-1(HO-1)抑制动脉粥样硬化进程的机制。方法:30只雄性SD大鼠随机分为正常对照组、模型组及氯血红素组。3月后观察主动脉形态学变化,ELISA法测定血中C-反应蛋白(CRP)及白介素-6(IL-6)水平,免疫组化法检测斑块内HO-1及巨噬细胞CD68的表达。结果:模型组及氯血红素组与对照组比较,CRPI、L-6、HO-1及CD68升高(P<0.05)。氯血红素组与模型组比较,主动脉内膜斑块面积占内膜面积比(RAAPI)显著减少(P<0.01),HO-1升高(P<0.01),CRPI、L-6及CD68降低(P<0.05)。结论:HO-1对动脉粥样硬化有明显的预防作用,这种作用可能是通过抑制炎症因子及巨噬细胞数量,减少炎症反应来实现的。

Effect of Heme Oxygenase-1 on Atherosclerosis in Rats

Objective: To explore the inhibition mechanism of heme oxygenase-1(HO-1) on the progress of atherosclerosis in rats.Methods: Thirty male SD rats were randomly divided into three groups as control group,model group and hemin group.After three months,the serum concentrations of C-reaction protein(CRP) and interleulin-6(IL-6) of each group were measured.The expression of HO-1 and CD68 was measured by immunenhistochemistry.Results: Compared with that respectively in the control group,the CRP,IL-6,HO-1 and CD-68 in the model group and the hemin group increased significantly(P<0.05).Compared with that in the model group,HO-1 of the hemin group increased significantly(P<0.01),while the RAAPI,MDA,CD68 and MMP-9 decreased(P<0.05).Conclusion: Heme oxygenase-1 can prevent atherosclerotic progression through inhibiting the inflammatory factors and decreasing the macrophages number,accordingly inhibiting the inflammatory response.