血小板衍生生长因子及其受体在肝纤维化患者肝组织中的表达及意义

目的：研究血小板衍生生长因子（PDGF）及受体（PDGFR）在慢性肝炎纤维化和肝硬化患者肝组织中的表达，分布和意义。方法：用免疫组织化学方法检测了21例慢性肝炎，42例肝硬化患者肝组织中PDGF－A，PDGF－B，PDGFR－α，PDGFR-β及α－平滑肌肌动蛋白（α－SMA）的表达，分布状况，定量和相关性分析。结果：慢性肝炎和肝硬化组织PDGF及其受体和α－SMA的表达主要分布于汇管，纤维间隔和炎细胞浸润聚集区，尤其见带分支突出的梭形细胞（活化的HSC）有大量表达。PDGF－B和PDGFR－β表达分别强于PDGF－A和PDGFR－α，两组间差异有显著性（P＜0．05－0．01）。α－SMA与PDGF－A，PDGF－B及PDGFR－α，PDGFR－β的表达，分布基本一致，定量分析呈正相关，相关系数分别为0．606（P＜0．001），0．772（P＜0．001），0．684（P＜0．001），0．825（P＜0．001）。结论：PDGF及其受体在肝纤维化发生发展中起重要作用，是通过活化HSC发挥效应，研究抑制PDGF及其受体的产生和作用是防治肝纤维化的新途径。

Expression and significance of platelet derived growth factor and its receptor in liver tissues of patients with liver fibrosis

OBJECTIVE: To study the expression and significance of platelet derived growth factor (PDGF) and its receptor (PDGFR) in liver tissues of patients with chronic hepatitis fibrosis and liver cirrhosis. METHODS: The expression, distribution, quantitation, and correlation of PDGF-A, PDGF-B, PDGFR-alpha, PDGFR-beta, and alpha-SMA in the liver tissues were analyzed by immunohistochemical techniques in 21 patients with chronic hepatitis and 42 patients with liver cirrhosis. RESULTS: In the liver tissues of chronic hepatitis and liver cirrhosis, PDGF and its receptor and alpha-SMA mainly distributed in the fibrotic septa and the infiltration area of inflammation, particularly in branch spindle-shaped cells (activated HSC). The expression of PDGF-B and PDGFR-beta was stronger than that of PDGF-A and PDGFR-alpha with a significant difference between them (P<0.05 approximately 0.01). The expression and distribution of alpha-SMA was basically identical with the expression and distribution of PDGF-A, PDGF-B and PDGFR-alpha, PDGFR-beta and quantitative analysis showed a positive correlation (r=0.606, P<0.001). CONCLUSIONS: PDGF and PDGFR play a key role in liver fibrogenesis and development. The biologic effects of PDGF are elicited through activising HSC. Inhibiting PDGF and its receptor is a new approach to the treatment of liver fibrosis.