人巨细胞病毒抑制粒-单及红系祖细胞的体外增殖及干预研究

探讨人巨细胞病毒 (HCMV)抑制脐血粒 -单系祖细胞的 (CFU GM)、红系爆式祖细胞 (BFU E)及红系祖细胞 (CFU E)的体外增殖和黄芪注射液与更昔洛韦(GCV)对此的干预作用 ,采用造血祖细胞体外培养、聚合酶链反应 (PCR)技术 ,培养、观察、计数CFU GM、BFU E及CFU E集落产率、抑制率、提高率 ,集落峰值时间和集落维持时间 ,并用PCR检测集落细胞内HCMV DNA。结果显示 :①HCMVAD169对CFU GM、BFU E及CFU E均有抑制作用 ,其抑制作用与HCMV浓度有关 ,高浓度 ( 1∶1 0 ,1 1 0 0 )感染组与对照组比较集落产率明显下降 (P <0 0 1 ) ,集落维持的时间明显缩短 (P <0 0 1 ) ,集落峰值时间无明显差异 (P >0 0 5) ,而低浓度组 ( 1∶1 0 0 0 )无此作用 ;②PCR检测发现在HCMV感染的造血祖细胞内存在HCMV DNA ;③在 1∶1 0感染组中 ,加入黄芪注射液与更昔洛韦后 ,HCMVAD169感染的CFU GM、BFU E及CFU E集落产率明显增加 (P <0 0 1 )。结论 :①造血祖细胞是HCMVAD169的宿主细胞之一 ,HCMVAD169能直接感染造血祖细胞 ;②在体外HCMVAD169对CFU GM、BFU E、CFU E的生长 ,集落形成有明显的抑制作用 ;③黄芪与更昔洛韦有抗HCMV作用 ,能促进受HCMV感染的造血祖细胞集落增殖

The suppression of HCMV on hepmato poietic progenitor and interfer research in vitro

To investigate the effect of human cytomegalovirus (HCMV) on the growth of cord blood granulocyte/monocyte progenitor and erythroid progenitor(CFU-GM and CFU-E),and astragalous and gancilovirs protect this, using the effect of HCMV AD 169 strain on hematopoietic stem cell growth was assay in vitro, HCMV AD 169 DNA in progenitors was detected by PCR in colony cell.The growth of CFU-GM? BFU-E and CFU-E were observed. Results:(1)HCMV AD 169 can suppress the growth of CFU-GM? BFU-E and CFU-E,this shows the decrease of colony number and colony lasting time.(2)The suppression have a relation to the dose of HCMV AD 169, significiant inhibition was observed at HCMV AD 169 dilution of 1∶10 and 1∶100,while at the dilution 1∶1000, this inhibition isn′t obvious.(3) HCMV-DNA was detected by PCR on nuclear of the hematopoietic progenitor.(4)In the group of HCMV AD 169 dilution of 1:10,the infected hematopoietic progenitors colony unmber increased significantly when astragalous and gancilovirs was applied. Conclusion HCMV AD 169 can suppression hematopoietic progenutor directly.Astragalous and gancilovirs can inhibit this suppression.