| 人前列腺癌细胞PC-3M亚系u-PAR基因与蛋白质表达 |
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| 引用本文： | 廖国宁,李清芬,冯友梅,邓耀祖.人前列腺癌细胞PC-3M亚系u-PAR基因与蛋白质表达[J].华中科技大学学报(医学版),2002,31(4):363-366. |
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| 作者姓名： | 廖国宁 李清芬 冯友梅 邓耀祖 |
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| 作者单位： | 1. 华中科技大学同济医学院基础医学院生物化学与分子生物学系,武汉,430030
2. 华中科技大学同济医学院基础医学院免疫学系,武汉,430030 |
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| 基金项目： | 湖北省自然科学基金资助项目 (No.2 0 0 0 J0 77) |
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| 摘 要： | 为研究与人前列腺癌细胞（PC-3M）侵袭能力相关的靶分子，采用有限稀释法分离单克隆细胞株，并应用单层细胞侵袭等实验鉴定各亚系的体外侵袭能力；借助RT-PCR和免疫组化的方法，分别在转录和翻译水平检测5株侵袭能力不同的PC-3M亚系尿激酶型纤溶酶原激活物受体（u-PAR)的表达。结果显示，高侵袭亚系-u-PAR基因mRNA的表达和蛋白质水平均明显高于低侵袭亚系，提示；PC-3M亚系u-PAR的高表达与其较强的侵袭能力密切相关，而u-PAR可能是抑制高侵袭亚系侵袭效应的一个重要靶分子。
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| 关 键 词： | 肿瘤侵袭 尿激酶型纤溶酶原激活物受体 前列腺肿瘤 细胞亚系 |
Analysis of the Expression of u-PAR at Mrna and Protein Levels in Differing Invasive PC-3M Cell Subclones |
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| Liao Guoning ,Li Qingfen ,Feng Youmei et al.Analysis of the Expression of u-PAR at Mrna and Protein Levels in Differing Invasive PC-3M Cell Subclones[J].Journal of Huazhong University of Science and Technology(Health Sciences),2002,31(4):363-366. |
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| Authors: | Liao Guoning Li Qingfen Feng Youmei |
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| Institution: | Liao Guoning 1,Li Qingfen 2,Feng Youmei 1 et al 1 Department of Biochemistry and Molecular Biology,2 Department of Immunology,School of Basic Medical Science,Tongji Medical College,Huazhong University of Science and Technology,Wuha |
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| Abstract: | To select a target molecule associated with invasive potential of PC 3M cell, we firstly isolated cell subclones from PC 3M cell line by using limited dilution method and characterized for their invasive potential by monolayer invasion assay. Then the expressions of u PAR at mRNA and protein levels in the cell subclones were examined by non competitive quantitative RT PCR and immunohistochemical assay, respectively. The results show that the higher content of u PAR was found in highly invasive cell subclones. It is suggested that the u PAR may be a good target molecule for inhibiting invasion by highly invasive PC 3M cell subclones. |
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| Keywords: | tumor invasion u PAR prostate carcinoma cell subclones |
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