| CRKL基因反义寡核苷酸对K562细胞增殖的影响 |
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| 引用本文： | 王莎,孙秉中,黄高升日,于文强,柴玉波. CRKL基因反义寡核苷酸对K562细胞增殖的影响[J]. 医学争鸣, 2001, 22(10): 878-880 |
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| 作者姓名： | 王莎 孙秉中 黄高升日 于文强 柴玉波 |
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| 作者单位： | 第四军医大学西京医院血液内科;第四军医大学基础部病理学教研室;第四军医大学基础部生物化学与分子生物学教研室 |
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| 基金项目： | 国家自然科学基金! ( 3 9770 3 3 6),陕西省科学基金 !( 98SM40) |
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| 摘 要： | 目的：通过研究CRKL基因反义寡核苷酸对K562细胞增残活性的影响,探讨CRKL基因在Ph^ 慢性粒细胞性白血病（CML）发病中的作用,方法： 脂质体为载体,CRKL基因反义寡核苷酸转染K562细胞,通过活细胸记数,H^3-TdR掺入,RT－PCR等方法观察K562细胞的增殖活性及基因表达的变化,结果,CRKL反义寡核苷酸对K562细胞的增殖有抑制作用,结论：CRKL基因在Ph^ CML的发病中可能有重要作用,可作为CML治疗的新靶点。
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| 关 键 词： | 反义寡核苷酸 CRKL基因 慢性粒细胞性白血病 |
| 文章编号： | 1000-2790(2001)10-0878-03 |
| 修稿时间： | 2000-11-13 |
Effect of CRKL gene expression on cell proliferation in K562 cell line |
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| WANG Sha,SUN Bing-Zhong,HUANG Gao-Sheng,YU Wen-qiang,CHAI Yu-bo. Effect of CRKL gene expression on cell proliferation in K562 cell line[J]. Negative, 2001, 22(10): 878-880 |
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| Authors: | WANG Sha SUN Bing-Zhong HUANG Gao-Sheng YU Wen-qiang CHAI Yu-bo |
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| Abstract: | AIM To investigate the effects of CRKL antisense oligonucleutides (ASODN) on cell growth and proliferation in K562 cell line and thus assess CRKL gene in the pathogenesis of Ph + CML. METHODS K562 cells were cultivated with CRKL oligos which were mediated by cationin liposome. The cell viability, proliferation, and gene expression were examined by H 3 TdR doping and RT PCR .SULTS CRKL ASODN could inhibit the viability and proliferation of K562 cells. CONCLUSION CRKL gene may play a key role in the pathogenisis in Ph + CML. |
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| Keywords: | antisense oligonucleutide CRKL gene chronic mylocytic leukemia |
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